Posttranscriptional chemical functionalization of azide-modified oligoribonucleotides by bioorthogonal click and Staudinger reactions

Direct incorporation of azide groups into RNA oligonucleotides by in vitro transcription reactions in the presence of a new azide-modified UTP analogue, and subsequent posttranscriptional chemical labeling of azide-modified oligoribonucleotide transcripts by click and Staudinger reactions are descri...

Full description

Saved in:
Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2012-01, Vol.48 (4), p.498-500
Main Authors: Rao, Harita, Sawant, Anupam A, Tanpure, Arun A, Srivatsan, Seergazhi G
Format: Article
Language:English
Subjects:
Analogue
Azides - chemistry
Base Sequence
Click Chemistry - methods
Marking
Modular
Oligonucleotides
Oligoribonucleotides - chemistry
Ribonucleic acids
Uridine Triphosphate - analogs & derivatives
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Direct incorporation of azide groups into RNA oligonucleotides by in vitro transcription reactions in the presence of a new azide-modified UTP analogue, and subsequent posttranscriptional chemical labeling of azide-modified oligoribonucleotide transcripts by click and Staudinger reactions are described. This postsynthetic labeling protocol is robust and modular, and offers an alternative access to RNA labeled with biophysical probes.
ISSN:1359-7345
1364-548X
DOI:10.1039/c1cc15659d