HvNax3—a locus controlling shoot sodium exclusion derived from wild barley (Hordeum vulgare ssp. spontaneum)

Previous work identified the wild barley (Hordeum vulgare ssp. spontaneum) accession CPI-71284-48 as being capable of limiting sodium (Na⁺) accumulation in the shoots under saline hydroponic growth conditions. Quantitative trait locus (QTL) analysis using a cross between CPI-71284-48 and a selection...

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Bibliographic Details
Published in:Functional & integrative genomics 2010-05, Vol.10 (2), p.277-291
Main Authors: Shavrukov, Yuri, Gupta, Narendra K, Miyazaki, Junji, Baho, Manahil N, Chalmers, Kenneth J, Tester, Mark, Langridge, Peter, Collins, Nicholas C
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Barley
Biochemistry
Bioinformatics
Biomedical and Life Sciences
Brachypodium
Cell Biology
Chromosome Mapping
Chromosomes, Plant - genetics
Crosses, Genetic
Cultivars
Gene expression
Genes, Plant - genetics
Genetic Markers
Genomics
H. vulgare ssp. spontaneum
Haploidy
Hordeum - genetics
Hordeum - metabolism
Hordeum vulgare
Life Sciences
Microbial Genetics and Genomics
Original Paper
Oryza - genetics
Oryza sativa
Plant Genetics and Genomics
Plant Shoots - genetics
Plant Shoots - metabolism
Proteins
Pyrophosphatase
Quantitative Trait Loci - genetics
Recombination, Genetic - genetics
Salinity
Salinity tolerance
Sodium - metabolism
Sodium transport
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Summary:Previous work identified the wild barley (Hordeum vulgare ssp. spontaneum) accession CPI-71284-48 as being capable of limiting sodium (Na⁺) accumulation in the shoots under saline hydroponic growth conditions. Quantitative trait locus (QTL) analysis using a cross between CPI-71284-48 and a selection of the cultivated barley (H. vulgare ssp. vulgare) cultivar Barque (Barque-73, a moderate Na⁺ excluder) attributed the control of the Na⁺ exclusion trait from CPI-71284-48 to a single locus on the short arm of chromosome 7H, which was named HvNax3. The locus reduced shoot Na⁺ accumulation by 10-25% in plants grown in 150 mM NaCl. Markers generated using colinearity with rice and Brachypodium, together with the analysis of introgression lines and F₂ and F₃ families, enabled HvNax3 to be mapped to a 1.3-cM interval. Genes from the corresponding rice and Brachypodium intervals encode 16 different classes of proteins and include several plausible candidates for HvNax3. The potential of HvNax3 to provide a useful trait contributing to salinity tolerance in cultivated barley is discussed.
ISSN:1438-793X
1438-7948
DOI:10.1007/s10142-009-0153-8