Axenic aerobic biofilms inhibit corrosion of SAE 1018 steel through oxygen depletion

Corrosion inhibition of SAE 1018 steel by pure-culture biofilms of Pseudomonas fragi and Escherichia coli DH5 alpha has been evaluated in complex Luria-Bertani medium, seawater-mimicking medium, and modified Baar's medium at 30 degrees C. In batch cultures, both bacteria inhibited corrosion thr...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 1997-07, Vol.48 (1), p.11-17
Main Authors: JAYARAMAN, A, CHENG, E. T, EARTHMAN, J. C, WOOD, T. K
Format: Article
Language:English
Subjects:
Aerobiosis
Anaerobiosis
Applied sciences
Bacteria
Bacterial corrosion
Biodeterioration. Biofouling
Biofilms
Biological and medical sciences
Biotechnology
Chemical analysis
Corrosion
Corrosion inhibitors
Corrosion mechanisms
Corrosion rate
Depletion
E coli
Electrochemistry
Escherichia coli
Escherichia coli - physiology
Exact sciences and technology
Fermentation
Fundamental and applied biological sciences. Psychology
Industrial applications and implications. Economical aspects
Low carbon steels
Metals. Metallurgy
Oxygen
Oxygen Consumption
Oxygen depletion
Pseudomonas - physiology
Pseudomonas fragi
Seawater
Steel
Steels
Water analysis
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Summary:Corrosion inhibition of SAE 1018 steel by pure-culture biofilms of Pseudomonas fragi and Escherichia coli DH5 alpha has been evaluated in complex Luria-Bertani medium, seawater-mimicking medium, and modified Baar's medium at 30 degrees C. In batch cultures, both bacteria inhibited corrosion three to six fold compared to sterile controls, and the corrosion was comparable to that observed in anaerobic sterile media. To corroborate this result, a continuous reactor and electrochemical impedance spectroscopy were used to show that both P. fragi K and E. coli DH5 alpha decreased the corrosion rate by 4- to 40-fold as compared to sterile controls; this matched the decrease in corrosion found with sterile medium in the absence of oxygen and with E. coli DH5 alpha grown anaerobically. In addition, the requirement for live respiring cells was demonstrated by the increase in the corrosion rate that was observed upon killing the P. fragi K biofilm in continuous cultures, and it was shown that fermentation products do not cause an increase in corrosion. Hence, pure-culture biofilms inhibit corrosion of SAE 1018 steel by depleting oxygen at the metal surface.
ISSN:0175-7598
1432-0614
DOI:10.1007/s002530051007