Rice gene expression profiles responding to larval feeding of the striped stem borer at the 1st to 2nd instar stage

• Published in: Insect science 2011-06, Vol.18 (3), p.273-281
• Main Authors: Sun, Yang, Zhang, Yong-Jun, Cao, Guang-Chun, Gu, Shao-Hua, Wu, Kong-Ming, Gao, Xi-Wu, Guo, Yu-Yuan
• Format: Article
• Language: English
• Subjects: allene; allene oxide cyclase (AOC); biosynthesis; Chilo suppressalis; diterpenoids; expressed sequence tags; gene expression; gene expression regulation; genes; Indexing in process; insects; instars; jasmonic acid; larvae; monoterpenoids; Oryza sativa; proteinase inhibitor (PI); proteinases; real-time quantitative PCR (RT-qPCR); reverse transcriptase polymerase chain reaction; rice; stress response; striped stem borer (SSB); suppression subtractive hybridization; suppression subtractive hybridization (SSH); terpene synthase (TPS); transcription (genetics); 喂养; 国家统计局; 幼虫; 抑制性消减杂交; 条纹; 水稻基因; 螟虫; 表达谱
• ISSN: 1672-9609; 1744-7917
• DOI: 10.1111/j.1744-7917.2010.01372.x

Abstract The objective of this study was to identify rice genes that are in response to the striped stem borer (SSB) (Chilo suppressalis Walker) feeding at the first to second larval stage. Using combined suppression subtractive hybridization (SSH) and dot blot approaches, we analyzed the induced defense genes that took place during the first 72 h of infesting intact rice (Oryza sativa L.) plants in sheath tissues with SSB larvae. By sequencing the whole SSH library, 39 expressed sequence tags involved in disease stress, insect stress or other stress responses were identified to be up-regulated by SSB larvae feeding. Among these genes, rice allene oxide cyclase (AOC), terpene synthase (TPS) and four proteinase inhibitor (PI) genes were up-regulated by SSB larvae feeding. Real-time quantitative reverse transcription polymerase chain reaction analysis showed that four rice PI genes were already up-regulated at 6 h, and reached peaks between 6 h to 12 h. In addition, the transcription of gene involving in jasmonate signaling pathway such as allene oxide cyclase (AOC) concerning rice early defense response to SSB feeding was activated after rice feeding by SSB for 2 h. Although the expression of rice terpene synthase (TPS) gene, involved in the biosynthesis of monoterpenes or diterpenes, was already up-regulated at 7 h, a significant increase in the expression was delayed until 12 h and reached its peak at 24 h. The present study identified six SSB-response genes and their expression patterns, which provides evidence and information to understand insect stress-response in plants.