A tetra-primer amplification refractory mutation system polymerase chain reaction for the evaluation of rs12979860 IL28B genotype

Recently, genome‐wide association studies in patients affected by HCV infection have identified a strong association between sustained virological response to peg‐interferon/ribavirin and spontaneous viral clearance and common single nucleotide polymorphisms (SNPs) near the IL28B gene, encoding for...

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Bibliographic Details
Published in:Journal of viral hepatitis 2011-09, Vol.18 (9), p.628-630
Main Authors: Galmozzi, E., Menico, B. Del, Rametta, R., Dongiovanni, P., Fracanzani, A. L., Benedan, L., Borroni, V., Maggioni, P., Fargion, S., Valenti , L.
Format: Article
Language:English
Subjects:
Adult
Aged
Alleles
Antiviral Agents - therapeutic use
chronic hepatitis C
Chronic infection
Data processing
Female
Genetic Testing - methods
genetics
Genotype
Hepacivirus - pathogenicity
Hepatitis
Hepatitis C virus
Hepatitis C, Chronic - drug therapy
Hepatitis C, Chronic - genetics
Hepatitis C, Chronic - virology
Humans
IL28
interferon lambda
Interferons
Interleukins - genetics
Interleukins - metabolism
Male
Middle Aged
Mutation
Polymerase chain reaction
Polymerase Chain Reaction - methods
Polymorphism, Single Nucleotide
Ribavirin
Ribavirin - therapeutic use
Single-nucleotide polymorphism
Treatment Outcome
treatment response
White People - genetics
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Description
Summary:Recently, genome‐wide association studies in patients affected by HCV infection have identified a strong association between sustained virological response to peg‐interferon/ribavirin and spontaneous viral clearance and common single nucleotide polymorphisms (SNPs) near the IL28B gene, encoding for interferon‐lambda‐3. Thus, it is anticipated that IL28B genotype determination will be integrated in clinical practice to guide treatment decisions. Here, we describe a simple tetra‐primer amplification refractory mutation system polymerase chain reaction (T‐ARMS‐PCR) for the evaluation of the rs12979860 C>T IL28B SNP, for which strong evidence of association with clinical outcomes has been collected in subjects of European descent. Valid genotypic data were obtained for over 99% of subjects analysed, and T‐ARMS‐PCR procedures were validated by the analysis of DNA samples of 164 patients with chronic HCV infection. In conclusion, this method allows rapid, reproducible, inexpensive and accurate detection of rs12979860 polymorphism without need of any special equipment and is also suitable for evaluation of a low number of samples on a routine basis.
ISSN:1352-0504
1365-2893
DOI:10.1111/j.1365-2893.2010.01349.x