Bovine Spongiform Encephalopathy: Investigation of Phenotypic Variation among Passive Surveillance Cases

Bovine spongiform encephalopathy (BSE) is a prion disease of domesticated cattle, first identified in Great Britain (GB) in 1986. The disease has been characterized by histopathological, immunohistochemical, biochemical and biological properties, which have shown a consistent disease phenotype among...

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Bibliographic Details
Published in:Journal of comparative pathology 2011-05, Vol.144 (4), p.277-288
Main Authors: Stack, M.J., Moore, S.J., Davis, A., Webb, P.R., Bradshaw, J.M., Lee, Y.H., Chaplin, M., Focosi-Snyman, R., Thurston, L., Spencer, Y.I., Hawkins, S.A.C., Arnold, M.E., Simmons, M.M., Wells, G.A.H.
Format: Article
Language:English
Subjects:
Animals
Biochemical characteristics
Biodiversity
Blotting, Western - veterinary
Bovine spongiform encephalopathy
Brain
Brain - metabolism
Brain - pathology
Cattle
Differentiation
Encephalopathy, Bovine Spongiform - metabolism
Encephalopathy, Bovine Spongiform - pathology
immunohistochemistry
Immunohistochemistry - veterinary
Phenotype
phenotype variation
Population Surveillance - methods
Prion protein
PrPSc Proteins - isolation & purification
PrPSc Proteins - metabolism
United Kingdom
Western blotting
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Summary:Bovine spongiform encephalopathy (BSE) is a prion disease of domesticated cattle, first identified in Great Britain (GB) in 1986. The disease has been characterized by histopathological, immunohistochemical, biochemical and biological properties, which have shown a consistent disease phenotype among cases obtained by passive surveillance. With the advent of active surveillance in 2001, immunological tests for detection of the prion protein revealed some cases with different biochemical characteristics and, in certain instances, differences in pathology that have indicated variant phenotypes and the possibility of agent strain variation. This study examines a case set of 523 bovine brains derived from archived material identified through passive surveillance in GB. All cases conformed to the phenotype of classical BSE (BSE-C) by histopathological, immunohistochemical and biochemical approaches. The analyses consolidated an understanding of BSE-C and, by western blotting, confirmed differentiation from the known atypical BSE cases which exhibit higher or lower molecular masses than BSE-C (BSE-H and BSE-L respectively).
ISSN:0021-9975
1532-3129
DOI:10.1016/j.jcpa.2010.10.007