Acute phase protein concentrations in colostrum-deprived pigs immunized with subunit and commercial vaccines against Glaesser's disease

• Published in: Veterinary immunology and immunopathology 2011-11, Vol.144 (1-2), p.61-67
• Main Authors: Martinez-Martinez, Sonia, Frandoloso, Rafael, Gutierrez-Martin, Cesar B, Lampreave, Fermin, Garcia-Iglesias, Maria Jose, Perez-Martinez, Claudia, Rodriguez-Ferri, Elias F
• Format: Article
• Language: English
• Subjects: Haemophilus
• ISSN: 0165-2427
• DOI: 10.1016/j.vetimm.2011.07.002

Haemophilus parasuisis the etiological agent of Glaesser's disease, which is characterized by fibrinous polyserositis, polyarthritis and meningitis in pigs. This study was focused on the characterization of the acute-phase response after immunization and infection of colostrum-deprived pigs with H. parasuisserovar 5, by measuring serum concentrations of three positive acute-phase proteins (APPs) (pig major acute-phase protein pig, MAP; haptoglobin, HPG; C-reactive protein, CRP) and one negative APP (apolipoprotein A-I, ApoA-I). Six experimental groups were established: a non-immunized but infected control group (CTL); two groups immunized with either a recombinant transferrin-binding protein (Tbp) A or TbpB fragment from H. parasuisNagasaki strain (rTbpA and rTbpB, respectively); two groups immunized with native outer membrane proteins with affinity to porcine transferrin (NPAPT), one of them inoculated intramuscularly (NPAPTim) and the other intratracheally (NPAPTit), and the last group receiving a commercially available bacterin (PG). The greatest concentrations of the three positive APPs and the lowest concentration of the negative APP were detected in CTL group, as well as in those animals belonging to rTbpA or rTbpB groups that died in response to challenge. Significant differences (P