Formulation and Drying of Alginate Beads for Controlled Release and Stabilization of Invertase

• Published in: Biomacromolecules 2011-09, Vol.12 (9), p.3147-3155
• Main Authors: Santagapita, Patricio R, Mazzobre, M. Florencia, Buera, M. Pilar
• Format: Article
• Language: English
• Subjects: Alginates - chemistry; Applied sciences; beta-Cyclodextrins - chemistry; beta-Fructofuranosidase - chemistry; beta-Fructofuranosidase - metabolism; Biological and medical sciences; Biotechnology; Calorimetry, Differential Scanning; Delayed-Action Preparations - chemistry; Delayed-Action Preparations - metabolism; Desiccation; Drug Carriers - chemistry; Drug Carriers - metabolism; Enzyme Stability; Exact sciences and technology; Freeze Drying; Fundamental and applied biological sciences. Psychology; Gels; Hydrogen-Ion Concentration; Immobilization of enzymes and other molecules; Immobilization techniques; Methods. Procedures. Technologies; Microscopy, Electron, Scanning; Natural polymers; Pectins - chemistry; Physicochemistry of polymers; Solubility; Starch and polysaccharides; Trehalose - chemistry
• ISSN: 1525-7797; 1526-4602
• DOI: 10.1021/bm2009075

Several alternatives to the conventional alginate beads formulation were studied for encapsulation of invertase. Pectin was added to the alginate/enzyme solution while trehalose and β-cyclodextrin were added to the calcium gelation media. The effect of composition changes, freezing, drying methods (freeze, vacuum, or air drying), and thermal treatment were evaluated on invertase stability and its release kinetics from beads. The enzyme release mechanism from wet beads depended on pH. The addition of trehalose, pectin, and β-cyclodextrin modified the bead structure, leading in some cases to a release mechanism that included the relaxation of the polymer chains, besides Fickian diffusion. Enzyme release from vacuum-dried beads was much faster than from freeze-dried beads, probably due to their higher pore size. The inclusion of β-cyclodextrin and especially of pectin prevented enzyme activity losses during bead generation, and trehalose addition was fundamental for achieving adequate invertase protection during freezing, drying, and thermal treatment. Present results showed that several alternatives such as drying method, composition, as well as pH of the relese medium can be managed to control enzyme release.