Proteomic analysis of zebrafish brain tissue following exposure to the pesticide prochloraz

• Published in: Aquatic toxicology 2011-10, Vol.105 (3), p.618-628
• Main Authors: Biales, Adam D., Bencic, David C., Villeneuve, Daniel L., Ankley, Gerald T., Lattier, David L.
• Format: Article
• Language: English
• Subjects: Animal, plant and microbial ecology; Animals; Applied ecology; aquatic environment; biochemical pathways; Biological and medical sciences; Biomarkers - metabolism; Brain; Brain - drug effects; Brain - metabolism; calcium; Chromatography, Liquid; Danio rerio; Ecotoxicology, biological effects of pollution; Electrophoresis, Gel, Two-Dimensional; Endocrine System - drug effects; Female; females; Fish Proteins - metabolism; Freshwater; Fundamental and applied biological sciences. Psychology; Fungicides, Industrial - toxicity; Gene expression; gene expression regulation; General aspects; Hormones - metabolism; Hypothalamo-Hypophyseal System - drug effects; Hypothalamus/pituitary/gonadal; Imidazoles - toxicity; learning; Male; males; Principal Component Analysis; Prochloraz; protein synthesis; proteins; proteome; Proteome - drug effects; Proteomics; Random Allocation; reproduction; Sex Factors; steroid hormones; Tandem Mass Spectrometry; unspecific monooxygenase; Water Pollutants, Chemical - toxicity; Zebrafish; Zebrafish - metabolism
• ISSN: 0166-445X; 1879-1514
• DOI: 10.1016/j.aquatox.2011.08.021

► Sex specific differences in gene expression profile in response to PCZ exposure. ► Neuroprotective, metabolic, Ca regulated and learning proteins were identified. ► Putative biomarker for PCZ exposure identified. ► Preliminary results demonstrate 100% accuracy in identifying PCZ exposed fish. The hypothalamus–pituitary–gonadal (HPG) axis plays a central role in the maintenance of homeostasis and disruptions in its function can have important implications for reproduction and other critical biological processes. A number of compounds found in aquatic environments are known to affect the HPG axis. In the present study, we used two-dimensional electrophoresis to investigate the proteome of female and male zebrafish brain after 96 h exposure to the fungicide prochloraz. Prochloraz has known effects on a number of key HPG molecules, including antagonism of Cyp17 and Cyp19 (aromatase). Twenty-eight proteins were shown to be differentially expressed in the brains of females and 22 in males. Proteins were identified using LC–MS/MS and identities were examined relative to brain function in the context of changing steroid hormone levels. There was little overlap between sexes in proteins exhibiting differential expression. Proteins with known roles in metabolism, learning, neuroprotection, and calcium regulation were determined to be differentially regulated. Relationships between identified proteins were also examined using Ingenuity Pathway Analysis, and females were shown to exhibit enrichment of several metabolic pathways. We used differentially expressed proteins to establish a putative classifier consisting of three proteins that was able to discriminate prochloraz-exposed from control females. Putatively impacted brain functions and specific protein changes that were observed have the potential to be generalized to other that similarly impact steroid hormone levels.