Dietary arginine and repeated handling increase disease resistance and modulate innate immune mechanisms of Senegalese sole ( Solea senegalensis Kaup, 1858)

• Published in: Fish & shellfish immunology 2011-12, Vol.31 (6), p.838-847
• Main Authors: Costas, Benjamín, Conceição, Luís E.C., Dias, Jorge, Novoa, Beatriz, Figueras, Antonio, Afonso, António
• Format: Article
• Language: English
• Subjects: Amino Acids - analysis; Animals; Arginine - pharmacology; Bacterial challenge; Diet; Dietary Supplements; Disease Resistance - drug effects; Disease Resistance - immunology; DNA Primers - genetics; Dose-Response Relationship, Drug; Fish Diseases - immunology; Flatfishes; Gene Expression Profiling - veterinary; Gram-Negative Bacterial Infections - immunology; Gram-Negative Bacterial Infections - veterinary; Handling (Psychology); Immune-related genes; Immunity, Innate - drug effects; Immunity, Innate - immunology; Nitric oxide; Photobacterium; Photobacterium damselae; Respiratory Burst; Respiratory burst activity; Solea senegalensis; Stress response; Stress, Physiological - immunology
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2011.07.024

Stress is known to impair immune function and disease resistance in fish. In the present study, repeated handling was employed as a chronic stressor in order to verify whether its attributed immunosuppressive effects could be minimized by dietary arginine supplementation. Therefore, Senegalese sole ( Solea senegalensis) were air exposed daily for 3 min during 14 days (handling) or left undisturbed (control). In addition, both control and handled specimens were fed 3 diets with graded levels of arginine (Arg 4.4, Arg 5.7 and Arg 6.9 g 16 g −1 N). Following the 14 days stress challenge and feeding on those diets, fish were infected with Photobacterium damselae subsp. piscicida (strain PC566.1; LD 50 5 × 10 3 cfu mL −1) and fed the same experimental diets. Respiratory burst activity and nitric oxide production of head–kidney leucocytes increased parallel to dietary arginine supplementation. HIF-1, HAMP-1, MIP1-alpha and gLYS expression values and some humoral parameters augmented in control specimens fed the Arg 5.7 and Arg 6.9 diets. Interestingly, repeated acute stress increased both disease resistance and some innate immune mechanisms in handled fish. The role of dietary arginine and repeated handling on Senegalese sole innate immunity and disease resistance are discussed. ► Dietary arginine supplementation enhanced some innate immune mechanisms and disease resistance in sole. ► Respiratory burst of head-kidney leucocytes increased parallel to dietary arginine supplementation. ► Repeated handling increased the levels of expression of HIF-1 and gLYS. ► Daily handling stress for 14 days increased disease resistance regardless of dietary treatment.