Gold nanoparticles surface-functionalized with paclitaxel drug and biotin receptor as theranostic agents for cancer therapy

Abstract We describe in this study whether the gold nanoparticle (AuNP) surface-functionalized with PEG, biotin, paclitaxel (PTX) and rhodamine B linked beta-cyclodextrin (β-CD) (AuNP- 5′ ) can be useful as a theranostic agent for cancer therapy without the cytotoxic effect on normal cells. Prior to...

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Bibliographic Details
Published in:Biomaterials 2012-01, Vol.33 (3), p.856-866
Main Authors: Heo, Dong Nyoung, Yang, Dae Hyeok, Moon, Ho-Jin, Lee, Jung Bok, Bae, Min Soo, Lee, Sang Cheon, Lee, Won Jun, Sun, In-Cheol, Kwon, Il Keun
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
Beta-cyclodextrin
beta-Cyclodextrins - chemistry
Biotin
Biotin - chemistry
Cell Line, Tumor
Cell Survival - drug effects
Dentistry
Flow Cytometry
Gold - chemistry
Gold nanoparticles
HeLa Cells
Humans
Metal Nanoparticles - chemistry
Mice
NIH 3T3 Cells
Paclitaxel
Paclitaxel - chemistry
Paclitaxel - pharmacology
Receptors, Growth Factor - chemistry
Theranostic agents
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Summary:Abstract We describe in this study whether the gold nanoparticle (AuNP) surface-functionalized with PEG, biotin, paclitaxel (PTX) and rhodamine B linked beta-cyclodextrin (β-CD) (AuNP- 5′ ) can be useful as a theranostic agent for cancer therapy without the cytotoxic effect on normal cells. Prior to surface-functionalizing AuNPs, the cytotoxicity of the nanoparticles was evaluated, followed by their cytocompatibility. PTX, an anti-cancer agent, formed inclusion complexations with β-CD conjugated AuNPs, and effectively released from the AuNP- 2′ surface-functionalized with PEG, beta-cyclodextrin (β-CD) and paclitaxel (PTX) using the intracellular glutathione (GSH) level (10 m m ). Two types of AuNP- 4 surface-functionalized with PEG and rhodamine B linked β-CD and AuNP- 5 surface-functionalized PEG, biotin and rhodamine B linked β-CD were used for evaluating their specific interaction on cancer cells such as HeLa, A549 and MG63. These were also tested against normal NIH3T3 cell, determining that the AuNP- 5 was more effectively involved with the cancer cells. Confocal laser scanning microscopy (CLSM), fluorescence-activated cell-sorting (FACS) and cell viability analyses showed that the AuNP- 5′ plays a significant role in the diagnosis and therapy of the cancer cells, and may be used in theranostic agents.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2011.09.064