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Self-assembled nanoparticles of hyaluronic acid/poly( dl-lactide-co-glycolide) block copolymer
We synthesized block copolymer composed of hyaluronic acid (HA) and poly( dl-lactide-co-glycolide) (PLGA) (HAbLG) for antitumor targeting. 1H NMR was employed to confirm synthesis of block copolymer. At 1H NMR study, HabLG nanoparticles showed HA intrinsic peaks only at D 2O, indicating that they co...
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| Published in: | Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2012-02, Vol.90, p.28-35 |
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| cited_by | cdi_FETCH-LOGICAL-c456t-faf5018441f6a857d9928b1629abb1fb52541ee5d8224864730ef0a0ade4579e3 |
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| cites | cdi_FETCH-LOGICAL-c456t-faf5018441f6a857d9928b1629abb1fb52541ee5d8224864730ef0a0ade4579e3 |
| container_end_page | 35 |
| container_issue | |
| container_start_page | 28 |
| container_title | Colloids and surfaces, B, Biointerfaces |
| container_volume | 90 |
| creator | Jeong, Young-Il Kim, Do Hyung Chung, Chung-Wook Yoo, Jin Ju Choi, Kyung Ha Kim, Cy Hyun Ha, Seung Hee Kang, Dae Hwan |
| description | We synthesized block copolymer composed of hyaluronic acid (HA) and poly(
dl-lactide-co-glycolide) (PLGA) (HAbLG) for antitumor targeting.
1H NMR was employed to confirm synthesis of block copolymer. At
1H NMR study, HabLG nanoparticles showed HA intrinsic peaks only at D
2O, indicating that they contained HA as a hydrophilic outer-shell and PLGA as a inner-core. Anti-tumor activity was studied using CD44-overexpressing HCT-116 human colon carcinoma cells. Addition of doxorubicin (DOX)-incorporated nanoparticles to tumor cells resulted in the expression of a strong red fluorescence color while they expressed very weak fluorescence when CD44 receptor was blocked with free HA. Flow cytometry data also showed similar results, indicating that the fluorescence intensity of tumor cells treated with nanoparticles was significantly decreased when CD44 receptor was blocked. These results indicate that HAbLG nanoparticles were able to target CD44-overexpressing tumor cells via receptor-mediated endocytosis. |
| doi_str_mv | 10.1016/j.colsurfb.2011.09.043 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_911163523</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0927776511005662</els_id><sourcerecordid>906559813</sourcerecordid><originalsourceid>FETCH-LOGICAL-c456t-faf5018441f6a857d9928b1629abb1fb52541ee5d8224864730ef0a0ade4579e3</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhi0EotvCXyi5UQ5Jbccf8Y2qgoJUiUPpFcuxx8WLEy92grT_Hq-25UhPo5Ged2Y0D0LnBHcEE3G57WyKZc1-7CgmpMOqw6x_gTZkkH3LeiFfog1WVLZSCn6CTkvZYowpI_I1OqEUY8K53KAfdxB9a0qBaYzgmtnMaWfyEmyE0iTf_NybuOY0B9sYG9zlLsX9ReNiG41dgoPWpvYh7us1tfnQjDHZX41NB2yC_Aa98iYWePtYz9D950_fr7-0t99uvl5f3baWcbG03niOycAY8cIMXDql6DASQZUZR-JHTjkjANwNlLJBMNlj8Nhg44BxqaA_Q--Pc3c5_V6hLHoKxUKMZoa0Fq0IIaLntH-exIJzNZADefFfkkiJ6UCFUhUVR9TmVEoGr3c5TCbvNcH64Etv9ZMvffClsdLVVw2eP-5Yxwncv9iToAq8OwLeJG0ecij6_q5OEFVmzxUmlfh4JKD-90-ArIsNMFtwIYNdtEvhuSv-Ahqxsus</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1770282699</pqid></control><display><type>article</type><title>Self-assembled nanoparticles of hyaluronic acid/poly( dl-lactide-co-glycolide) block copolymer</title><source>Elsevier</source><creator>Jeong, Young-Il ; Kim, Do Hyung ; Chung, Chung-Wook ; Yoo, Jin Ju ; Choi, Kyung Ha ; Kim, Cy Hyun ; Ha, Seung Hee ; Kang, Dae Hwan</creator><creatorcontrib>Jeong, Young-Il ; Kim, Do Hyung ; Chung, Chung-Wook ; Yoo, Jin Ju ; Choi, Kyung Ha ; Kim, Cy Hyun ; Ha, Seung Hee ; Kang, Dae Hwan</creatorcontrib><description>We synthesized block copolymer composed of hyaluronic acid (HA) and poly(
dl-lactide-co-glycolide) (PLGA) (HAbLG) for antitumor targeting.
1H NMR was employed to confirm synthesis of block copolymer. At
1H NMR study, HabLG nanoparticles showed HA intrinsic peaks only at D
2O, indicating that they contained HA as a hydrophilic outer-shell and PLGA as a inner-core. Anti-tumor activity was studied using CD44-overexpressing HCT-116 human colon carcinoma cells. Addition of doxorubicin (DOX)-incorporated nanoparticles to tumor cells resulted in the expression of a strong red fluorescence color while they expressed very weak fluorescence when CD44 receptor was blocked with free HA. Flow cytometry data also showed similar results, indicating that the fluorescence intensity of tumor cells treated with nanoparticles was significantly decreased when CD44 receptor was blocked. These results indicate that HAbLG nanoparticles were able to target CD44-overexpressing tumor cells via receptor-mediated endocytosis.</description><identifier>ISSN: 0927-7765</identifier><identifier>EISSN: 1873-4367</identifier><identifier>DOI: 10.1016/j.colsurfb.2011.09.043</identifier><identifier>PMID: 22001557</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Antineoplastic Agents - chemistry ; Antineoplastic Agents - metabolism ; Antineoplastic Agents - pharmacology ; Binding, Competitive ; Block copolymer ; Block copolymers ; Blocking ; Carcinoma - drug therapy ; Carcinoma - immunology ; Carcinoma - pathology ; CD44 ; Cell Line, Tumor ; colloids ; Colonic Neoplasms - drug therapy ; Colonic Neoplasms - immunology ; Colonic Neoplasms - pathology ; color ; colorectal neoplasms ; composite polymers ; Delayed-Action Preparations - chemistry ; Delayed-Action Preparations - metabolism ; deuterium oxide ; Deuterium Oxide - analysis ; Deuterium Oxide - metabolism ; doxorubicin ; Doxorubicin - chemistry ; Doxorubicin - metabolism ; Doxorubicin - pharmacology ; Drug Carriers - chemistry ; Drug Carriers - metabolism ; Endocytosis ; Flow Cytometry ; Fluorescence ; Humans ; Hyaluronan Receptors - immunology ; Hyaluronan Receptors - metabolism ; Hyaluronic acid ; Hyaluronic Acid - chemistry ; hydrophilicity ; Hydrophobic and Hydrophilic Interactions ; Hydroxyapatite ; Lactic Acid - chemistry ; Magnetic Resonance Spectroscopy ; Molecular Targeted Therapy - methods ; Nanoparticles ; neoplasm cells ; nuclear magnetic resonance spectroscopy ; PLGA ; Polyglycolic Acid - chemistry ; Receptor-mediated endocytosis ; Receptors ; Tumors</subject><ispartof>Colloids and surfaces, B, Biointerfaces, 2012-02, Vol.90, p.28-35</ispartof><rights>2011 Elsevier B.V.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-faf5018441f6a857d9928b1629abb1fb52541ee5d8224864730ef0a0ade4579e3</citedby><cites>FETCH-LOGICAL-c456t-faf5018441f6a857d9928b1629abb1fb52541ee5d8224864730ef0a0ade4579e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22001557$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jeong, Young-Il</creatorcontrib><creatorcontrib>Kim, Do Hyung</creatorcontrib><creatorcontrib>Chung, Chung-Wook</creatorcontrib><creatorcontrib>Yoo, Jin Ju</creatorcontrib><creatorcontrib>Choi, Kyung Ha</creatorcontrib><creatorcontrib>Kim, Cy Hyun</creatorcontrib><creatorcontrib>Ha, Seung Hee</creatorcontrib><creatorcontrib>Kang, Dae Hwan</creatorcontrib><title>Self-assembled nanoparticles of hyaluronic acid/poly( dl-lactide-co-glycolide) block copolymer</title><title>Colloids and surfaces, B, Biointerfaces</title><addtitle>Colloids Surf B Biointerfaces</addtitle><description>We synthesized block copolymer composed of hyaluronic acid (HA) and poly(
dl-lactide-co-glycolide) (PLGA) (HAbLG) for antitumor targeting.
1H NMR was employed to confirm synthesis of block copolymer. At
1H NMR study, HabLG nanoparticles showed HA intrinsic peaks only at D
2O, indicating that they contained HA as a hydrophilic outer-shell and PLGA as a inner-core. Anti-tumor activity was studied using CD44-overexpressing HCT-116 human colon carcinoma cells. Addition of doxorubicin (DOX)-incorporated nanoparticles to tumor cells resulted in the expression of a strong red fluorescence color while they expressed very weak fluorescence when CD44 receptor was blocked with free HA. Flow cytometry data also showed similar results, indicating that the fluorescence intensity of tumor cells treated with nanoparticles was significantly decreased when CD44 receptor was blocked. These results indicate that HAbLG nanoparticles were able to target CD44-overexpressing tumor cells via receptor-mediated endocytosis.</description><subject>Antineoplastic Agents - chemistry</subject><subject>Antineoplastic Agents - metabolism</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Binding, Competitive</subject><subject>Block copolymer</subject><subject>Block copolymers</subject><subject>Blocking</subject><subject>Carcinoma - drug therapy</subject><subject>Carcinoma - immunology</subject><subject>Carcinoma - pathology</subject><subject>CD44</subject><subject>Cell Line, Tumor</subject><subject>colloids</subject><subject>Colonic Neoplasms - drug therapy</subject><subject>Colonic Neoplasms - immunology</subject><subject>Colonic Neoplasms - pathology</subject><subject>color</subject><subject>colorectal neoplasms</subject><subject>composite polymers</subject><subject>Delayed-Action Preparations - chemistry</subject><subject>Delayed-Action Preparations - metabolism</subject><subject>deuterium oxide</subject><subject>Deuterium Oxide - analysis</subject><subject>Deuterium Oxide - metabolism</subject><subject>doxorubicin</subject><subject>Doxorubicin - chemistry</subject><subject>Doxorubicin - metabolism</subject><subject>Doxorubicin - pharmacology</subject><subject>Drug Carriers - chemistry</subject><subject>Drug Carriers - metabolism</subject><subject>Endocytosis</subject><subject>Flow Cytometry</subject><subject>Fluorescence</subject><subject>Humans</subject><subject>Hyaluronan Receptors - immunology</subject><subject>Hyaluronan Receptors - metabolism</subject><subject>Hyaluronic acid</subject><subject>Hyaluronic Acid - chemistry</subject><subject>hydrophilicity</subject><subject>Hydrophobic and Hydrophilic Interactions</subject><subject>Hydroxyapatite</subject><subject>Lactic Acid - chemistry</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Molecular Targeted Therapy - methods</subject><subject>Nanoparticles</subject><subject>neoplasm cells</subject><subject>nuclear magnetic resonance spectroscopy</subject><subject>PLGA</subject><subject>Polyglycolic Acid - chemistry</subject><subject>Receptor-mediated endocytosis</subject><subject>Receptors</subject><subject>Tumors</subject><issn>0927-7765</issn><issn>1873-4367</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqFkU1v1DAQhi0EotvCXyi5UQ5Jbccf8Y2qgoJUiUPpFcuxx8WLEy92grT_Hq-25UhPo5Ged2Y0D0LnBHcEE3G57WyKZc1-7CgmpMOqw6x_gTZkkH3LeiFfog1WVLZSCn6CTkvZYowpI_I1OqEUY8K53KAfdxB9a0qBaYzgmtnMaWfyEmyE0iTf_NybuOY0B9sYG9zlLsX9ReNiG41dgoPWpvYh7us1tfnQjDHZX41NB2yC_Aa98iYWePtYz9D950_fr7-0t99uvl5f3baWcbG03niOycAY8cIMXDql6DASQZUZR-JHTjkjANwNlLJBMNlj8Nhg44BxqaA_Q--Pc3c5_V6hLHoKxUKMZoa0Fq0IIaLntH-exIJzNZADefFfkkiJ6UCFUhUVR9TmVEoGr3c5TCbvNcH64Etv9ZMvffClsdLVVw2eP-5Yxwncv9iToAq8OwLeJG0ecij6_q5OEFVmzxUmlfh4JKD-90-ArIsNMFtwIYNdtEvhuSv-Ahqxsus</recordid><startdate>20120201</startdate><enddate>20120201</enddate><creator>Jeong, Young-Il</creator><creator>Kim, Do Hyung</creator><creator>Chung, Chung-Wook</creator><creator>Yoo, Jin Ju</creator><creator>Choi, Kyung Ha</creator><creator>Kim, Cy Hyun</creator><creator>Ha, Seung Hee</creator><creator>Kang, Dae Hwan</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><scope>7QO</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20120201</creationdate><title>Self-assembled nanoparticles of hyaluronic acid/poly( dl-lactide-co-glycolide) block copolymer</title><author>Jeong, Young-Il ; Kim, Do Hyung ; Chung, Chung-Wook ; Yoo, Jin Ju ; Choi, Kyung Ha ; Kim, Cy Hyun ; Ha, Seung Hee ; Kang, Dae Hwan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-faf5018441f6a857d9928b1629abb1fb52541ee5d8224864730ef0a0ade4579e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Antineoplastic Agents - chemistry</topic><topic>Antineoplastic Agents - metabolism</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Binding, Competitive</topic><topic>Block copolymer</topic><topic>Block copolymers</topic><topic>Blocking</topic><topic>Carcinoma - drug therapy</topic><topic>Carcinoma - immunology</topic><topic>Carcinoma - pathology</topic><topic>CD44</topic><topic>Cell Line, Tumor</topic><topic>colloids</topic><topic>Colonic Neoplasms - drug therapy</topic><topic>Colonic Neoplasms - immunology</topic><topic>Colonic Neoplasms - pathology</topic><topic>color</topic><topic>colorectal neoplasms</topic><topic>composite polymers</topic><topic>Delayed-Action Preparations - chemistry</topic><topic>Delayed-Action Preparations - metabolism</topic><topic>deuterium oxide</topic><topic>Deuterium Oxide - analysis</topic><topic>Deuterium Oxide - metabolism</topic><topic>doxorubicin</topic><topic>Doxorubicin - chemistry</topic><topic>Doxorubicin - metabolism</topic><topic>Doxorubicin - pharmacology</topic><topic>Drug Carriers - chemistry</topic><topic>Drug Carriers - metabolism</topic><topic>Endocytosis</topic><topic>Flow Cytometry</topic><topic>Fluorescence</topic><topic>Humans</topic><topic>Hyaluronan Receptors - immunology</topic><topic>Hyaluronan Receptors - metabolism</topic><topic>Hyaluronic acid</topic><topic>Hyaluronic Acid - chemistry</topic><topic>hydrophilicity</topic><topic>Hydrophobic and Hydrophilic Interactions</topic><topic>Hydroxyapatite</topic><topic>Lactic Acid - chemistry</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Molecular Targeted Therapy - methods</topic><topic>Nanoparticles</topic><topic>neoplasm cells</topic><topic>nuclear magnetic resonance spectroscopy</topic><topic>PLGA</topic><topic>Polyglycolic Acid - chemistry</topic><topic>Receptor-mediated endocytosis</topic><topic>Receptors</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jeong, Young-Il</creatorcontrib><creatorcontrib>Kim, Do Hyung</creatorcontrib><creatorcontrib>Chung, Chung-Wook</creatorcontrib><creatorcontrib>Yoo, Jin Ju</creatorcontrib><creatorcontrib>Choi, Kyung Ha</creatorcontrib><creatorcontrib>Kim, Cy Hyun</creatorcontrib><creatorcontrib>Ha, Seung Hee</creatorcontrib><creatorcontrib>Kang, Dae Hwan</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jeong, Young-Il</au><au>Kim, Do Hyung</au><au>Chung, Chung-Wook</au><au>Yoo, Jin Ju</au><au>Choi, Kyung Ha</au><au>Kim, Cy Hyun</au><au>Ha, Seung Hee</au><au>Kang, Dae Hwan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Self-assembled nanoparticles of hyaluronic acid/poly( dl-lactide-co-glycolide) block copolymer</atitle><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle><addtitle>Colloids Surf B Biointerfaces</addtitle><date>2012-02-01</date><risdate>2012</risdate><volume>90</volume><spage>28</spage><epage>35</epage><pages>28-35</pages><issn>0927-7765</issn><eissn>1873-4367</eissn><abstract>We synthesized block copolymer composed of hyaluronic acid (HA) and poly(
dl-lactide-co-glycolide) (PLGA) (HAbLG) for antitumor targeting.
1H NMR was employed to confirm synthesis of block copolymer. At
1H NMR study, HabLG nanoparticles showed HA intrinsic peaks only at D
2O, indicating that they contained HA as a hydrophilic outer-shell and PLGA as a inner-core. Anti-tumor activity was studied using CD44-overexpressing HCT-116 human colon carcinoma cells. Addition of doxorubicin (DOX)-incorporated nanoparticles to tumor cells resulted in the expression of a strong red fluorescence color while they expressed very weak fluorescence when CD44 receptor was blocked with free HA. Flow cytometry data also showed similar results, indicating that the fluorescence intensity of tumor cells treated with nanoparticles was significantly decreased when CD44 receptor was blocked. These results indicate that HAbLG nanoparticles were able to target CD44-overexpressing tumor cells via receptor-mediated endocytosis.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>22001557</pmid><doi>10.1016/j.colsurfb.2011.09.043</doi><tpages>8</tpages></addata></record> |
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| ispartof | Colloids and surfaces, B, Biointerfaces, 2012-02, Vol.90, p.28-35 |
| issn | 0927-7765 1873-4367 |
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| source | Elsevier |
| subjects | Antineoplastic Agents - chemistry Antineoplastic Agents - metabolism Antineoplastic Agents - pharmacology Binding, Competitive Block copolymer Block copolymers Blocking Carcinoma - drug therapy Carcinoma - immunology Carcinoma - pathology CD44 Cell Line, Tumor colloids Colonic Neoplasms - drug therapy Colonic Neoplasms - immunology Colonic Neoplasms - pathology color colorectal neoplasms composite polymers Delayed-Action Preparations - chemistry Delayed-Action Preparations - metabolism deuterium oxide Deuterium Oxide - analysis Deuterium Oxide - metabolism doxorubicin Doxorubicin - chemistry Doxorubicin - metabolism Doxorubicin - pharmacology Drug Carriers - chemistry Drug Carriers - metabolism Endocytosis Flow Cytometry Fluorescence Humans Hyaluronan Receptors - immunology Hyaluronan Receptors - metabolism Hyaluronic acid Hyaluronic Acid - chemistry hydrophilicity Hydrophobic and Hydrophilic Interactions Hydroxyapatite Lactic Acid - chemistry Magnetic Resonance Spectroscopy Molecular Targeted Therapy - methods Nanoparticles neoplasm cells nuclear magnetic resonance spectroscopy PLGA Polyglycolic Acid - chemistry Receptor-mediated endocytosis Receptors Tumors |
| title | Self-assembled nanoparticles of hyaluronic acid/poly( dl-lactide-co-glycolide) block copolymer |
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