Peptide derived from Pvfp-1 as bioadhesive on bio-inert surface

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2012-02, Vol.90, p.227-235
• Main Authors: Jiang, Zhen, Yu, Yabiao, Du, Lina, Ding, Xiyu, Xu, Hui, Sun, Yanan, Zhang, Qiqing
• Format: Article
• Language: English
• Subjects: Adhesion; Adsorption; Amino Acid Sequence; Animals; Bio-inert surface; Bioadhesive; Biochemistry; Biocompatibility; Biomedical materials; Cell Adhesion - drug effects; Cell Line; Cell Proliferation - drug effects; Coated Materials, Biocompatible - chemistry; Coated Materials, Biocompatible - metabolism; Coated Materials, Biocompatible - pharmacology; Coating; Dihydroxyphenylalanine - chemistry; DOPA; Mice; Molecular Sequence Data; Mussels; Osteoblasts - cytology; Osteoblasts - drug effects; Peptide; Peptides; Peptides - chemistry; Peptides - metabolism; Peptides - pharmacology; Perna - chemistry; Perna viridis; Polytetrafluoroethylene - chemistry; Polytetrafluoroethylene - metabolism; Polytetrafluoroethylenes; Protein Structure, Tertiary; PTFE; Surface chemistry; Surface Properties; Tissue Adhesives - chemistry; Tissue Adhesives - metabolism; Tissue Adhesives - pharmacology; Tissue Engineering - methods; Tissue Scaffolds
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2011.10.037

[Display omitted] ► Peptide C2 (M) showed outstanding adsorption and adhesion ability especially on the bio-inert PTFE. ► DOPA amount and position, and the hydrophobicity of peptide could affect adsorption and adhesion. ► C2 (M) coated well on PTFE and improved cell adhesion and cell viability on PTFE and was non-toxic. ► C2 (M) improves the bio-activity of PTFE, and it would be used as bioadhesive on bio-inert materials. Surface property is one important characteristic of materials, especially for ones that are bio-inert but designed for bio-medical application. In this study, we designed a series of peptides and compared their capacities as bioadhesive to improve the surface bioactivity of bio-inert material. The peptides were designed according to the sequence of Perna viridis foot protein 1 (Pvfp-1), one of the Mfp-1s (mussel foot protein 1) which play key roles in wet adhesion of mussel byssus. And the Teflon (PTFE) was chosen as a model of bio-inert material. With adsorption, adhesion and coating analysis, it was found that peptide C2 (M) (derived from the non-repeating region of Pvfp-1, contains modified DOPA) has superior coating and adhesion abilities especially on the bio-inert surface of PTFE. After coating with peptide C2 (M), the cell adhesion and spreading of osteoblast MC3T3-E1 cells on PTFE were significantly improved compared with those on non-coated surface, and the peptide-coating did not show any cell toxicity. Therefore, peptide C2 (M) is effective for improving the bioactivity of bio-inert PTFE, and could be potentially used as a bioadhesive on other bio-inert materials for biomedical application. Moreover, this study also provided new insights in designing other peptide-based bioadhesive materials.