Quantitative analysis of biofilm formation of methicillin-resistant Staphylococcus aureus (MRSA) strains from patients with orthopaedic device-related infections

• Published in: FEMS immunology and medical microbiology 2011-10, Vol.63 (1), p.10-15
• Main Authors: Kawamura, Hideki, Nishi, Junichiro, Imuta, Naoko, Tokuda, Koichi, Miyanohara, Hiroaki, Hashiguchi, Teruto, Zenmyo, Michihisa, Yamamoto, Takuya, Ijiri, Kosei, Kawano, Yoshifumi, Komiya, Setsuro
• Format: Article
• Language: English
• Subjects: Bacterial Proteins - genetics; Bacteriology; biofilm; Biofilms; Biofilms - growth & development; Biological and medical sciences; Carrier State - microbiology; Colonization; Drug resistance; Electrophoresis; Electrophoresis, Gel, Pulsed-Field; Epidemiology; Fundamental and applied biological sciences. Psychology; Gel electrophoresis; Genotype; Genotyping; Humans; Infection; Laboratory equipment; medical device; Medical devices; Medical electronics; Medical equipment; Methicillin; Methicillin-Resistant Staphylococcus aureus - classification; Methicillin-Resistant Staphylococcus aureus - genetics; Methicillin-Resistant Staphylococcus aureus - isolation & purification; Methicillin-Resistant Staphylococcus aureus - physiology; Microbiology; Miscellaneous; Molecular Typing; MRSA; orthopaedics; Orthopedics; Patients; Prosthesis-Related Infections - microbiology; Pulsed-field gel electrophoresis; Quantitative analysis; Staphylococcal Infections - microbiology; Staphylococcus aureus; Staphylococcus infections; Strains (organisms); Trans-Activators - genetics
• ISSN: 0928-8244; 1574-695X; 2049-632X
• DOI: 10.1111/j.1574-695X.2011.00821.x

Abstract Biofilms play a pivotal role in medical device-related infections. However, epidemiological analysis of biofilm formation and genotyping among clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients with orthopaedic infections has rarely been reported. A total of 168 MRSA strains were examined: 23 strains from patients with device-related infection (the device group); 55 from patients with device-non-related infection (the nondevice group); and 90 from asymptomatic nasal carriers (the colonization group). Pulsed-field gel electrophoresis analysis and five genotyping methods including agr typing were performed. Biofilm formation was quantified using a microtitre plate assay. The device group had a significantly higher incidence of agr-2 than the colonization group (78.3% vs. 34.4%, P=0.001). The biofilm index of the agr-2 (0.523 ± 0.572) strains was significantly higher than those of agr-1 (0.260 ± 0.418, P