A polymer nanostructured Fabry–Perot interferometer based biosensor

► Polymer nanostructured Fabry–Perot interferometer (FPI) based biosensor. ► The nanostructured FPI has a layer of Au-coated nanopores inside its cavity. ► Its detection limit is lower than 10 pg/mL for immunoglobulin G and protein A binding. A polymer nanostructured Fabry–Perot interferometer (FPI)...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2011-12, Vol.30 (1), p.128-132
Main Authors: Zhang, Tianhua, Pathak, Pushparaj, Karandikar, Sukrut, Giorno, Rebecca, Que, Long
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensing
Biosensing Techniques - instrumentation
Biosensors
Biotechnology
Equipment Design
Equipment Failure Analysis
Fabry–Perot interferometer
Fundamental and applied biological sciences. Psychology
Immunoglobulin G - analysis
Immunoglobulin G - chemistry
Interferometry - instrumentation
Localized surface plasmon resonance effect
Methods. Procedures. Technologies
Nanostructures
Nanostructures - chemistry
Polymers - chemistry
Staphylococcal Protein A - chemistry
Various methods and equipments
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Summary:► Polymer nanostructured Fabry–Perot interferometer (FPI) based biosensor. ► The nanostructured FPI has a layer of Au-coated nanopores inside its cavity. ► Its detection limit is lower than 10 pg/mL for immunoglobulin G and protein A binding. A polymer nanostructured Fabry–Perot interferometer (FPI) based biosensor is reported. Different from a conventional FPI, the nanostructured FPI has a layer of Au-coated nanopores inside its cavity. The Au-coated nanostructure layer offers significant enhancement of optical transducing signals due to the localized surface plasmon resonance effect and also due to the significantly increased sensing surface area, which is up to at least two orders of magnitude larger than that of a conventional FPI-based biosensor. Using this technical platform, the immobilization of captures proteins (protein A) on the nanostructure layer and their binding with immunoglobulin G (IgG) has been monitored in real time, resulting in the shift of the interference fringes of the optical transducing signals. Current results show that the limit-of-detection of the biosensor should be lower than 10 pg/mL for IgG-protein A binding.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2011.08.042