A Novel Monoclonal Antibody against Human CD80 and its Immune Protection in a Mouse Lupus-like Disease

Blockade of the interactions between CD28/CTLA-4 and their ligands, CD80 (B7, B7.1)/CD86 (B70, B7.2), is an attractive means to induce antigen-specific peripheral tolerance in autoimmune disease and organ transplantation. In this study, we generated and characterized a monoclonal antibody (Clone 4E5...

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Bibliographic Details
Published in:International journal of immunopathology and pharmacology 2011-07, Vol.24 (3), p.583-593
Main Authors: Shi, Q., Gao, Z.Y., Xie, F., Wang, L.F., Gu, Y.P., Yang, T.J., Huang, L., Qian, Q.H., Qiu, Y.H.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Antinuclear - pharmacology
Antibodies, Monoclonal - chemistry
Antibodies, Monoclonal - pharmacology
Antibodies, Monoclonal - therapeutic use
Antigens, CD - biosynthesis
Antigens, CD - genetics
B7-1 Antigen - immunology
CD28 Antigens - drug effects
CD28 Antigens - physiology
Cell Line
Cell Proliferation - drug effects
Disease Models, Animal
Flow Cytometry
Fluorescent Antibody Technique
Humans
Immunosuppressive Agents
Kidney - immunology
Lupus Erythematosus, Systemic - chemically induced
Lupus Erythematosus, Systemic - drug therapy
Lupus Nephritis - chemically induced
Lupus Nephritis - pathology
Lymphocyte Culture Test, Mixed
Mice
Mice, Inbred C57BL
Signal Transduction - drug effects
Spleen - cytology
Spleen - drug effects
T-Lymphocytes - drug effects
Terpenes
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Summary:Blockade of the interactions between CD28/CTLA-4 and their ligands, CD80 (B7, B7.1)/CD86 (B70, B7.2), is an attractive means to induce antigen-specific peripheral tolerance in autoimmune disease and organ transplantation. In this study, we generated and characterized a monoclonal antibody (Clone 4E5) against human CD80. 4E5 could recognize both human and mouse CD80 and suppress mixed lymphocyte reaction in vitro. To investigate their potency for clinical use, we further administrated 4E5 to a mouse lupus-like disease model (C57BL/J6) induced by Pristane. 4E5 could inhibit the immune response and attenuate the severity of lupus-like disease. The data showed 4E5 function and suggested that blockade of CD80/CD28 co-stimulatory signal pathway with 4E5 is a promising strategy to decelerate the progression of lupus-like disease and other autoimmune diseases.
ISSN:0394-6320
2058-7384
DOI:10.1177/039463201102400304