Poly(ethylene oxide) Layers Grafted to Dopamine-melanin Anchoring Layer: Stability and Resistance to Protein Adsorption

In this study, we propose substrate-independent modification for creating a protein-repellent surface based on dopamine-melanin anchoring layer used for subsequent binding of poly(ethylene oxide) (PEO) from melt. We verified that the dopamine-melanin layer can be formed on literally any substrate an...

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Bibliographic Details
Published in:Biomacromolecules 2011-09, Vol.12 (9), p.3232-3242
Main Authors: Pop-Georgievski, Ognen, Popelka, Štěpán, Houska, Milan, Chvostová, Dagmar, Proks, Vladimír, Rypáček, František
Format: Article
Language:English
Subjects:
Adsorption
Animals
Applied sciences
Biosensing Techniques - methods
Blood Proteins - chemistry
Blood Proteins - metabolism
Cattle
Chromatography, High Pressure Liquid
Coated Materials, Biocompatible - analysis
Coated Materials, Biocompatible - chemical synthesis
Exact sciences and technology
Humans
Hydrophobic and Hydrophilic Interactions
Melanins - chemistry
Microscopy, Atomic Force
Microscopy, Electron, Transmission
Organic polymers
Physicochemistry of polymers
Polyethylene Glycols - chemistry
Polymers with particular properties
Preparation, kinetics, thermodynamics, mechanism and catalysts
Protein Binding
Surface Properties
Tissue Engineering - methods
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Summary:In this study, we propose substrate-independent modification for creating a protein-repellent surface based on dopamine-melanin anchoring layer used for subsequent binding of poly(ethylene oxide) (PEO) from melt. We verified that the dopamine-melanin layer can be formed on literally any substrate and could serve as the anchoring layer for subsequent grafting of PEO chains. Grafting of PEO from melt in a temperature range 70–110 °C produces densely packed PEO layers showing exceptionally low protein adsorption when exposed to the whole blood serum or plasma. The PEO layers prepared from melt at 110 °C retained the protein repellent properties for as long as 10 days after their exposure to physiological-like conditions. The PEO-dopamine-melanin modification represents a simple and universal surface modification method for the preparation of protein repellent surfaces that could serve as a nonfouling background in various applications, such as optical biosensors and tissue engineering.
ISSN:1525-7797
1526-4602
DOI:10.1021/bm2007086