Identification of Mycobacterium bovis among mycobacterial isolates from human clinical specimens at a university hospital in Rio de Janeiro, Brazil

In 2005 and 2006, 8,121 clinical specimens submitted to the Mycobacteriology Laboratory of the Clementino Fraga Filho University Hospital/Thoracic Diseases Institute, in the city of Rio de Janeiro, Brazil, were inoculated on Löwenstein-Jensen medium containing glycerol and pyruvate. There were 79 my...

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Bibliographic Details
Published in:Jornal brasileiro de pneumologia 2011-09, Vol.37 (5), p.664-668
Main Authors: Sobral, Luciana Fonseca, Duarte, Rafael Silva, Vieira, Gisele Betzler de Oliveira, Silva, Marlei Gomes da, Boechat, Neio, Fonseca, Leila de Souza
Format: Article
Language:English
Subjects:
Bacteriological Techniques - methods
Brazil - epidemiology
Culture Media - chemistry
Hospitals, University
Humans
Mycobacterium bovis - growth & development
Mycobacterium tuberculosis - classification
Mycobacterium tuberculosis - isolation & purification
Tuberculosis - epidemiology
Tuberculosis - microbiology
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Summary:In 2005 and 2006, 8,121 clinical specimens submitted to the Mycobacteriology Laboratory of the Clementino Fraga Filho University Hospital/Thoracic Diseases Institute, in the city of Rio de Janeiro, Brazil, were inoculated on Löwenstein-Jensen medium containing glycerol and pyruvate. There were 79 mycobacteria isolates that presented growth only on pyruvate-containing medium, and those isolates were selected for the presumptive identification of Mycobacterium bovis. The selected isolates were screened with biochemical tests, PCR amplification (with the specific primers Rv0577 and Rv1510), and pyrazinamide susceptibility tests. All of the strains isolated showed specific phenotypical and genotypical patterns characteristic of M. tuberculosis, and no M. bovis strains were detected.
ISSN:1806-3756
DOI:10.1590/s1806-37132011000500015