Separation strategies for untargeted metabolomics

Metabolomics has rapidly become a profiling technique of choice for biomarker elucidation and molecular diagnostics in addition to studies focused on understanding disease pathogenesis. Key to the success of metabolomics in these areas has been the techniques to separate and analyze the chemically d...

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Bibliographic Details
Published in:Journal of separation science 2011-12, Vol.34 (24), p.3460-3469
Main Author: Patti, Gary J
Format: Article
Language:English
Subjects:
Biological and medical sciences
biomarkers
Carbohydrates - isolation & purification
Chromatography
Chromatography, Liquid
Criteria
Diagnostic systems
Global
Humans
Investigative techniques, diagnostic techniques (general aspects)
Mass Spectrometry
Medical sciences
Metabolic profiling
metabolites
metabolome
Metabolomics
Metabolomics - methods
Miscellaneous. Technology
Optimization
Pathogenesis
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Profiling
Separation
Strategy
Untargeted
Workflow
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Summary:Metabolomics has rapidly become a profiling technique of choice for biomarker elucidation and molecular diagnostics in addition to studies focused on understanding disease pathogenesis. Key to the success of metabolomics in these areas has been the techniques to separate and analyze the chemically diverse group of compounds comprising the metabolome by using global and untargeted approaches. Untargeted metabolomic efforts have the goal of examining as many metabolites as possible simultaneously and most frequently use an LC/MS‐based approach. Here, the importance of LC in an untargeted metabolomic workflow is outlined and separation strategies for optimization are reviewed within the context of these criteria.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201100532