The Expression Analysis of Notch-1 and Jagged-2 During the Development of the Hindgut in Rat Embryos with Ethylenethiourea Induced Anorectal Malformations

Background This study was designed to investigate the expression of Notch-1 and Jagged-2 in the terminal hindgut in ethylenethiourea (ETU)-exposed rat embryos with anorectal malformations (ARMs) and its potential association with the maldevelopment of the terminal hindgut in ARMs. Material and Metho...

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Bibliographic Details
Published in:The Journal of surgical research 2012, Vol.172 (1), p.131-136
Main Authors: Jia, Huimin, M.D, Chen, Qingjiang, M.D, Zhang, Tao, M.D, Bai, Yuzuo, M.D., Ph.D, Yuan, Zhengwei, M.D., Ph.D, Wang, Weilin, M.D., Ph.D
Format: Article
Language:English
Subjects:
Animals
Anorectal Malformations
Anus, Imperforate - chemically induced
Anus, Imperforate - metabolism
development
Ethylenethiourea - adverse effects
Gastrointestinal Tract - embryology
Gastrointestinal Tract - metabolism
Gene Expression Regulation, Developmental
Jagged-2 Protein
Male
Membrane Proteins - metabolism
Models, Animal
notch
Rats
Rats, Wistar
Receptor, Notch1 - metabolism
RNA, Messenger - metabolism
Surgery
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Summary:Background This study was designed to investigate the expression of Notch-1 and Jagged-2 in the terminal hindgut in ethylenethiourea (ETU)-exposed rat embryos with anorectal malformations (ARMs) and its potential association with the maldevelopment of the terminal hindgut in ARMs. Material and Methods ETU-exposed ARMs model was introduced to investigate the expression pattern of Notch-1 and Jagged-2 during the hindgut development using immunohistochemical staining, reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot analysis. Results Immunostaining revealed that the expression of Notch-1 and Jagged-2 showed changes in the developing terminal hindgut of ARMs. The expression of Notch-1 and Jagged-2 in the terminal hindgut of ARMs rat embryos decreased at both mRNA level and protein level ( P < 0.05) compared with normal tissues. Conclusion This study demonstrated that the expression of Notch-1 and Jagged-2 in ARMs of ETU-exposed rat embryos was remarkably reduced, which implied its potential role in the pathogenesis of the terminal hindgut maldevelopment in ARMs.
ISSN:0022-4804
1095-8673
DOI:10.1016/j.jss.2010.08.011