Interconversion Between Intestinal Stem Cell Populations in Distinct Niches

Intestinal epithelial stem cell identity and location have been the subject of substantial research. Cells in the +4 niche are slow-cycling and label-retaining, whereas a different stem cell niche located at the crypt base is occupied by crypt base columnar (CBC) cells. CBCs are distinct from +4 cel...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2011-12, Vol.334 (6061), p.1420-1424
Main Authors: Takeda, Norifumi, Jain, Rajan, LeBoeuf, Matthew R., Wang, Qiaohong, Lu, Min Min, Epstein, Jonathan A.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Cycle
Cell Differentiation
Cell Lineage
Cell Proliferation
Cells
Cells, Cultured
Crypts
Cultured cells
Descendants
Endocrine cells
Epithelial cells
Epithelial Cells - cytology
Fundamental and applied biological sciences. Psychology
Homeodomain Proteins - analysis
Homeodomain Proteins - genetics
Intestinal Mucosa - cytology
Intestinal Mucosa - drug effects
Intestine, Small - cytology
Intestine, Small - drug effects
Intestine. Mesentery
Intestines
Mice
Models, Biological
Multipotent Stem Cells - cytology
Multipotent Stem Cells - physiology
Neural stem cells
Organoids
Paneth Cells - cytology
Stem Cell Niche
Stem cells
Tamoxifen - pharmacology
Vertebrates: digestive system
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Summary:Intestinal epithelial stem cell identity and location have been the subject of substantial research. Cells in the +4 niche are slow-cycling and label-retaining, whereas a different stem cell niche located at the crypt base is occupied by crypt base columnar (CBC) cells. CBCs are distinct from +4 cells, and the relationship between them is unknown, though both give rise to all intestinal epithelial lineages. We demonstrate that Hopx, an atypical homeobox protein, is a specific marker of +4 cells. Hopx-expressing cells give rise to CBCs and all mature intestinal epithelial lineages. Conversely, CBCs can give rise to +4 Hopx-positive cells. These findings demonstrate a bidirectional lineage relationship between active and quiescent stem cells in their niches.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1213214