Coupling Electrodeposition with Layer-by-Layer Assembly to Address Proteins within Microfluidic Channels

Two thin‐film assembly methods are coupled to address proteins. Electrodeposition confers programmability and generates a template for layer‐by‐layer (LbL) assembly. LbL enables precise control of film thickness and the incorporation of labile biological components. The capabilities are demonstrated...

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Bibliographic Details
Published in:Advanced materials (Weinheim) 2011-12, Vol.23 (48), p.5817-5821
Main Authors: Wang, Yifeng, Liu, Yi, Cheng, Yi, Kim, Eunkyoung, Rubloff, Gary W., Bentley, William E., Payne, Gregory F.
Format: Article
Language:English
Subjects:
alginate
Alginates - chemistry
Algorithms
Animals
Aspergillus niger - metabolism
Brachyura
chitosan
Chitosan - chemistry
electroaddressing
Electrochemistry - methods
electrodeposition
Electroplating - methods
Equipment Design
Glucose Oxidase - chemistry
Glucuronic Acid - chemistry
Hexuronic Acids - chemistry
layer-by-layer assembly
Lipid Bilayers - chemistry
Macrocystis - metabolism
Materials Testing
Microfluidics - methods
Microscopy - methods
Microscopy, Fluorescence - methods
Polymers - chemistry
Spectrum Analysis, Raman - methods
Surface Properties
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Summary:Two thin‐film assembly methods are coupled to address proteins. Electrodeposition confers programmability and generates a template for layer‐by‐layer (LbL) assembly. LbL enables precise control of film thickness and the incorporation of labile biological components. The capabilities are demonstrated using glucose oxidase (GOx) based electrochemical biosensing within a microfabricated fluidic device.
ISSN:0935-9648
1521-4095
DOI:10.1002/adma.201103726