Protein-loaded PLGA–PEG–PLGA microspheres: A tool for cell therapy

A promising strategy to repair injured organs is possible by delivering a growth factor via poly-( d, l lactide- co-glycolide) (PLGA) microspheres; the latter are coated with adhesion molecules that serve as a support for cell delivery. At present, PLGA is not the optimal choice of polymer because o...

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Bibliographic Details
Published in:European journal of pharmaceutical sciences 2012-01, Vol.45 (1), p.128-137
Main Authors: Tran, Van-Thanh, Karam, Jean-Pierre, Garric, Xavier, Coudane, Jean, Benoît, Jean-Pierre, Montero-Menei, Claudia N., Venier-Julienne, Marie-Claire
Format: Article
Language:English
Subjects:
Adsorption
Biological and medical sciences
Biomimetic Materials - chemistry
Biomimetic Materials - metabolism
Bone Marrow Cells - cytology
Bone Marrow Cells - metabolism
Cell Adhesion
Cell Survival
Cells, Cultured
Controlled protein release
Fibronectins - chemistry
Fibronectins - metabolism
General pharmacology
Humans
Hydrogen-Ion Concentration
Hydrophobic and Hydrophilic Interactions
Lysozyme
Medical sciences
Microspheres
Molecular Weight
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
PLGA–PEG–PLGA
Poloxamer - chemistry
Poloxamer 188
Polyethylene Glycols - chemistry
Polyethylene Glycols - metabolism
Polyglactin 910 - chemistry
Polyglactin 910 - metabolism
Protein Denaturation
Solubility
Stromal Cells - cytology
Stromal Cells - metabolism
Surface Properties
Tissue Engineering - methods
Tissue Scaffolds - chemistry
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Summary:A promising strategy to repair injured organs is possible by delivering a growth factor via poly-( d, l lactide- co-glycolide) (PLGA) microspheres; the latter are coated with adhesion molecules that serve as a support for cell delivery. At present, PLGA is not the optimal choice of polymer because of poor or incomplete protein release. The use of a more hydrophilic PLGA–PEG–PLGA (A–B–A) copolymer increases the degree of protein release. In this work, the impact of different combinations of (B) and (A) segments on the protein-release profile has been investigated. Continuous-release profiles, with no lag phases, were observed. The triblock ABA with a low molecular weight of PEG and a high molecular weight of PLGA showed an interesting release pattern with a small burst (
ISSN:0928-0987
1879-0720
DOI:10.1016/j.ejps.2011.10.030