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Roles of hinge region, loops 3 and 4 in the activation of Escherichia coli cyclic AMP receptor protein

The cAMP receptor protein (CRP) requires cAMP for an allosteric change and regulates more than 150 genes in Escherichia coli. In this study, the modular half of cAMP receptor protein was used to investigate the allosteric signal transmission pathway induced by cAMP binding. The activation of CRP upo...

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Published in:	International journal of biological macromolecules 2012-01, Vol.50 (1), p.1-6
Main Authors:	Gao, Zhengya, Li, Feng, Wu, Guangrong, Zhu, Yanrun, Yu, Ting, Yu, Shaoning
Format:	Article
Language:	English
Subjects:	adenosine monophosphate Amino Acid Motifs Calorimetry - methods cAMP cAMP binding motif cAMP receptor protein Chymotrypsin - chemistry Circular Dichroism Conformational change cyclic AMP Cyclic AMP Receptor Protein - chemistry Cyclic AMP Receptor Protein - metabolism Dimerization Escherichia coli Escherichia coli - metabolism Escherichia coli Proteins - chemistry genes Kinetics Macromolecular Substances - chemistry Protein Binding Protein Conformation Protein Structure, Tertiary Signal Transduction Spectrometry, Fluorescence - methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Temperature
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container_title	International journal of biological macromolecules
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creator	Gao, Zhengya Li, Feng Wu, Guangrong Zhu, Yanrun Yu, Ting Yu, Shaoning
description	The cAMP receptor protein (CRP) requires cAMP for an allosteric change and regulates more than 150 genes in Escherichia coli. In this study, the modular half of cAMP receptor protein was used to investigate the allosteric signal transmission pathway induced by cAMP binding. The activation of CRP upon cAMP binding is indicated to be realignment of the two subunits within the CRP dimer. The interaction of loop 3 and Phe136 do not involve in signal transmission.
doi_str_mv	10.1016/j.ijbiomac.2011.08.016
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subjects	adenosine monophosphate Amino Acid Motifs Calorimetry - methods cAMP cAMP binding motif cAMP receptor protein Chymotrypsin - chemistry Circular Dichroism Conformational change cyclic AMP Cyclic AMP Receptor Protein - chemistry Cyclic AMP Receptor Protein - metabolism Dimerization Escherichia coli Escherichia coli - metabolism Escherichia coli Proteins - chemistry genes Kinetics Macromolecular Substances - chemistry Protein Binding Protein Conformation Protein Structure, Tertiary Signal Transduction Spectrometry, Fluorescence - methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Temperature
title	Roles of hinge region, loops 3 and 4 in the activation of Escherichia coli cyclic AMP receptor protein
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