2-Aminoethoxydiphenyl borate (2-APB) reduces respiratory burst, MMP-9 release and CD11b expression, and increases l-selectin shedding in bovine neutrophils

• Published in: Research in veterinary science 2012-02, Vol.92 (1), p.103-110
• Main Authors: Conejeros, I., Jara, E., Carretta, M.D., Alarcón, P., Hidalgo, M.A, Burgos, R.A.
• Format: Article
• Language: English
• Subjects: 2-APB; Animals; Apoptosis - drug effects; Boron Compounds - administration & dosage; Boron Compounds - pharmacology; Calcium - metabolism; Calcium Channel Blockers - administration & dosage; Calcium Channel Blockers - pharmacology; Calcium Signaling - drug effects; Cattle - metabolism; CD11b; CD11b Antigen - metabolism; Cells; Conflicts of interest; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel - veterinary; Flow Cytometry - veterinary; Kinases; l-Selectin; L-Selectin - metabolism; Matrix Metalloproteinase 9 - metabolism; MMP-9; Neutrophils; Neutrophils - drug effects; Neutrophils - metabolism; Platelet Activating Factor - metabolism; Proteins; Reactive Oxygen Species - metabolism; Respiratory Burst - drug effects; Superoxide anion; Veterinary medicine
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2010.10.005

This study describes the effect of 2-aminoethoxydiphenyl borate (2-APB), a putative store-operated calcium (Ca 2+) entry (SOCE) inhibitor, on reactive oxygen species (ROS) production, matrix metalloproteinase 9 (MMP-9) release, CD11b and l-selectin (CD62L) expression, size changes and apoptosis in bovine neutrophils stimulated with platelet-activating factor (PAF). It was observed that doses ⩾1 μM 2-APB significantly reduced ROS production, whereas 50 and 100 μM 2-APB reduced MMP-9 release induced by PAF. Moreover, concentrations ⩾10 μM 2-APB reduced CD11b expression and increased l-selectin shedding. PAF induced size changes in neutrophils, and this effect was inhibited by 2-APB. From this work it is possible to conclude that 2-APB at concentrations that inhibit SOCE responses was able to inhibit ROS and MMP-9 release and CD11b expression, and increase l-selectin shedding, suggesting that the Ca 2+ channel involved in SOCE is a potential target for the development of new anti-inflammatory drugs in cattle.