Genetic Characterization of Protostrongylus shiozawai From Japanese Serows (Capricornis crispus)

Protostrongylus shiozawai (Nematoda: Strongylida) infection is known to occur in the lungs of wild Japanese serows (Capricornis crispus) and, to date, has been classified only by its morphological characteristics, as well as host specificity. To characterize P. shiozawai genetically, a partial seque...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of parasitology 2011-12, Vol.97 (6), p.1178-1180
Main Authors: Murakami, M, Inoshima, Y, El-Dakhly, Kh M, Yanai, T, Ishiguro, N
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biological taxonomies
Capricornis crispus
Classification
DNA
DNA, Helminth - chemistry
DNA, Intergenic - chemistry
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Genetic markers
Host specificity
Host-Parasite Interactions
Infection
Infections
Invertebrates
Lung
Lung - parasitology
Lung - pathology
Lung Diseases, Parasitic - parasitology
Lung Diseases, Parasitic - pathology
Lung Diseases, Parasitic - veterinary
Lungs
Metastrongyloidea - classification
Metastrongyloidea - genetics
Molecular Sequence Data
Morphology
Nematoda
Nematodes
Parasitology
Phylogenetics
Phylogeny
Polymerase chain reaction
RESEARCH NOTES
Ruminants - parasitology
Sequence Alignment
Sheep
Spacer
Strongylida
Strongylida Infections - parasitology
Strongylida Infections - pathology
Strongylida Infections - veterinary
Veterinary medicine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Protostrongylus shiozawai (Nematoda: Strongylida) infection is known to occur in the lungs of wild Japanese serows (Capricornis crispus) and, to date, has been classified only by its morphological characteristics, as well as host specificity. To characterize P. shiozawai genetically, a partial sequence of the second internal transcribed spacer (ITS-2) region was determined and compared with those of related nematodes. Our subsequent classification of P. shiozawai based on phylogenetic analysis was consistent with the current classification. Phylogenetic analysis also showed that P. shiozawai is more closely related to Protostrongylus stilesi than to Protostrongylus rufescens. PCR using genetic markers in the ITS-2 region should provide a useful tool for expanded studies of P. shiozawai and other Protostrongylus species.
ISSN:0022-3395
1937-2345
DOI:10.1645/GE-2830.1