Characterization of sodium hyaluronate blends using frit inlet asymmetrical flow field-flow fractionation and multiangle light scattering

We characterized ultrahigh molecular weight sodium hyaluronate (NaHA) and blended pharmaceutical products containing NaHA using flow field-flow fractionation and multiangle light scattering–differential refractive index (FlFFF-MALS-DRI). NaHA is a water-soluble polysaccharide with a range of molecul...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2012, Vol.402 (3), p.1269-1276
Main Authors: Ali, Muhammad, Hwang, Euijin, Cho, Il-Hwan, Moon, Myeong Hee
Format: Article
Language:English
Subjects:
Analytical Chemistry
Biochemistry
Blends
Cellulose
Characterization and Evaluation of Materials
Chemical properties
Chemistry
Chemistry and Materials Science
Food Science
Fractionation
Fractionation, Field Flow - methods
Hyaluronic acid
Hyaluronic Acid - chemistry
Inlets
Laboratory Medicine
Light
Light scattering
Molecular weight
Monitoring/Environmental Analysis
Original Paper
Pharmaceutical Preparations - chemistry
Pharmaceuticals
Polymer blends
Refractometry
Scattering, Radiation
Sodium
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Summary:We characterized ultrahigh molecular weight sodium hyaluronate (NaHA) and blended pharmaceutical products containing NaHA using flow field-flow fractionation and multiangle light scattering–differential refractive index (FlFFF-MALS-DRI). NaHA is a water-soluble polysaccharide with a range of molecular weights (MW; 10 5 ~10 8  Da) that is found in body fluids and tissues. NaHA is also used commercially in pharmaceutical and cosmetic applications. We used a frit inlet asymmetrical FlFFF channel to separate aqueous polymers according to their hydrodynamic size, and we used on-line measurements of light scattering to obtain the MW distribution (MWD) as well as structural information about NaHA in aqueous solution. In this study, we investigated NaHA and anti-adhesive blend mixtures of NaHA (a commercial NaHA blend mixture containing sodium carboxymethyl cellulose and a new blend with hydroxyethyl starch (HES)) to determine the molecular weight distribution MWD of NaHA and the blend mixtures and to obtain structural information about these compounds in aqueous solution. We also examined the characteristics of NaHA–HES–polylactic- co -glycolic acid film products exposed to gamma radiation for sterilization purposes.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-011-5531-0