Molecular cloning, tissue distribution and ontogenetic expression of Xiang pig Chemerin and its involvement in regulating energy metabolism through Akt and ERK1/2 signaling pathways

• Published in: Molecular biology reports 2012-02, Vol.39 (2), p.1887-1894
• Main Authors: Yang, Huansheng, Li, Fengna, Kong, Xiangfeng, Yuan, Xiaoxue, Lian, Guoqi, Geng, Meimei, Li, Tiejun, Yin, Jingdong, Yin, Yulong
• Format: Article
• Language: English
• Subjects: Adipokines - genetics; Adipokines - metabolism; Adipokines - pharmacology; Age Factors; Amino acids; Analysis of Variance; Animal Anatomy; Animal Biochemistry; Animals; Bioenergetics; Biomedical and Life Sciences; Blotting, Western; Cell Line, Tumor; Chemotactic Factors - genetics; Chemotactic Factors - metabolism; Cloning; Cloning, Molecular; Computational Biology; Energy Metabolism - drug effects; Energy Metabolism - physiology; Gene Expression; Gene Expression Profiling; Histology; Insulin; Kidney; Kinases; Life Sciences; Lung; MAP Kinase Signaling System - physiology; metabolic disorders; Metabolism; Mice; Molecular biology; Morphology; Muscles; obesity; Open Reading Frames - genetics; Reverse Transcriptase Polymerase Chain Reaction; rodents; Signal transduction; Sus scrofa - genetics
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-011-0934-8

Chemerin, as a new member of adipokines family, is highly expressed in adipose tissue in rodent and its expression increases with obesity. Moreover, chemerin has been reported to have significant relationship with metabolic syndrome and insulin sensitivity. Here, the gene encoding chemerin from Xiang pig was cloned. The open reading frame of this cDNA encodes 163 deduced amino acid residues. The putative protein has a N-terminal signaling peptide and a nuclear localization signal profile which are highly conserved among the vertebrate orthologs. Both chemerin and chemerinR are highly expressed in lung, kidney and small intestine in adult Xiang pig. Besides these tissues, chemerin is abundant in liver and backfat, and chemerinR is abundant in spleen and skeletal muscle. We also investigated the age-dependent expression of chemerin in suckling Xiang piglets in various tissues, which showed an interaction between age and segments in abundance of chemerin and chemerinR from day 1 to day 21. For chemerinR, it was abundant in skeletal muscle of both adult and fetal Xiang pig. Further, we treated differentiated C2C12 cells with chemerin. The result showed that chemerin regulated energy metabolism partly through Akt and ERK1/2 signaling pathway. Taken together, our findings provide basic molecular information for the deeper investigation on the function of chemerin.