Clinical Application of MAGE A1-6 RT-Nested PCR for Diagnosis of Lung Cancer Invisible by Bronchoscopy

• Published in: Anticancer research 2012, Vol.32 (1), p.163-167
• Main Authors: SHIN, Kyeong-Cheol, EUN YOUNG CHOI, JIN HONG CHUNG, JEON, Changho, KWAN HO LEE
• Format: Article
• Language: English
• Subjects: Adenocarcinoma - diagnosis; Adenocarcinoma - genetics; Adult; Aged; Aged, 80 and over; Antigens, Neoplasm - genetics; Biological and medical sciences; Biomarkers, Tumor - genetics; Bronchoalveolar Lavage; Bronchoscopy; Carcinoma, Large Cell - diagnosis; Carcinoma, Large Cell - genetics; Carcinoma, Non-Small-Cell Lung - diagnosis; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Squamous Cell - diagnosis; Carcinoma, Squamous Cell - genetics; Female; Follow-Up Studies; Humans; Lung Neoplasms - diagnosis; Lung Neoplasms - genetics; Male; Medical sciences; Melanoma-Specific Antigens - genetics; Middle Aged; Neoplasm Proteins - genetics; Pneumology; Polymerase Chain Reaction; Prognosis; Prospective Studies; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; Small Cell Lung Carcinoma - diagnosis; Small Cell Lung Carcinoma - genetics; Tumors; Tumors of the respiratory system and mediastinum
• ISSN: 0250-7005; 1791-7530

The main goal of this study was to evaluate the diagnostic efficacy of melanoma-associated antigen (MAGE) A1-6 reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) of bronchial washing fluid for the detection of lung cancer invisible by bronchoscopy. To determine the expression of MAGE A1-6 gene in 75 lung carcinomas diagnosed by conventional fluoroscopy-guided lung biopsy and 58 cancer-free controls, RT-nested PCR was performed of bronchial washing fluid. MAGE A1-6 RT-nested PCR data was analyzed according to tumor histology, stage, size, and compared with cytological data. MAGE A1-6 RT-nested PCR displayed higher sensitivity (64.0%) than that of conventional cytology (14.7%). There was no significant correlation between MAGE gene expression and histological types or clinical stage. For tumor size, detection rates were 74.0% in tumor smaller than 3 cm and 58.7% in these larger than 3 cm. MAGE A1-6 RT-nested PCR of bronchial washing fluid may be a useful method for diagnosis of peripheral lung cancer in clinical practice.