Development of SCAR marker for simultaneous identification of Miscanthus sacchariflorus, M. sinensis and M. x giganteus

The sequence-characterized amplified region (SCAR) marker for simultaneous identification of Miscanthus sacchariflorus , Miscanthus sinensis , and Miscanthus x giganteus was developed. In this study, it was attempted for the first time to develop the SCAR marker for detecting the molecular phenotype...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2012, Vol.35 (1-2), p.55-59
Main Authors: Kim, Jung Kon, An, Gi Hong, Ahn, Seung-Hyun, Moon, Youn-Ho, Cha, Young-Lok, Bark, Surn-Teh, Choi, Yong-Hwan, Suh, Sae-Jung, Seo, Sang-Gyu, Kim, Sun-Hyung, Koo, Bon-Cheol
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomarkers
Biotechnology
Chemistry
Chemistry and Materials Science
Deoxyribonucleic acid
DNA
DNA, Plant - analysis
DNA, Plant - genetics
Environmental Engineering/Biotechnology
Food Science
Fundamental and applied biological sciences. Psychology
Genetic Markers - genetics
Genotype & phenotype
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Original Paper
Poaceae - classification
Poaceae - genetics
Polymerase Chain Reaction - methods
Random Amplified Polymorphic DNA Technique - methods
Species Specificity
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Summary:The sequence-characterized amplified region (SCAR) marker for simultaneous identification of Miscanthus sacchariflorus , Miscanthus sinensis , and Miscanthus x giganteus was developed. In this study, it was attempted for the first time to develop the SCAR marker for detecting the molecular phenotypes among Miscanthus species. Randomly amplified polymorphic DNA technique was applied for this study and one fragment which is unique to M. sacchariflorus was identified and then sequenced. Based on the specific fragment, one SCAR primer pair designated as MS62-5F and MS62-5R was designed to amplify an approximately 1,000 bp DNA fragment within the sequenced region. Diagnostic PCR was performed using the primer pair. Using this SCAR marker, approximately 1,000 bp and 1,200 bp DNA fragments were obtained in M. sacchariflorus and M. sinensis , respectively. Moreover, M. x giganteus was obtained both bands at the same time. The result showed that this SCAR marker can clearly distinguish the M. sacchariflorus , M. sinensis , and M. x giganteus , respectively.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-011-0592-1