Improved high-pressure enzymatic biodiesel batch synthesis in near-critical carbon dioxide

The enzymatic synthesis of biodiesel by a high-pressure semi-continuous process in near-critical carbon dioxide (NcCO 2 ) was studied. Biodiesel synthesis was evaluated in both batch and semi-continuous systems to develop an effective process. Batch processing demonstrated the advantageous propertie...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2012, Vol.35 (1-2), p.105-113
Main Authors: Lee, Myunggu, Lee, Dohoon, Cho, Jin Ku, Cho, Jaehoon, Han, Jinmi, Park, Chulhwan, Kim, Sangyong
Format: Article
Language:English
Subjects:
Bioconversions. Hemisynthesis
Biodiesel fuels
Biofuel production
Biofuels - analysis
Biological and medical sciences
Biotechnology
Canola oil
Carbon dioxide
Carbon Dioxide - chemical synthesis
Chemical synthesis
Chemistry
Chemistry and Materials Science
Energy
Environmental Engineering/Biotechnology
Enzymes
Food Science
Fundamental and applied biological sciences. Psychology
Industrial and Production Engineering
Industrial applications and implications. Economical aspects
Industrial Chemistry/Chemical Engineering
Lipase - chemistry
Lipase - classification
Methods. Procedures. Technologies
Original Paper
Plant Oils - chemistry
Pressure
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Summary:The enzymatic synthesis of biodiesel by a high-pressure semi-continuous process in near-critical carbon dioxide (NcCO 2 ) was studied. Biodiesel synthesis was evaluated in both batch and semi-continuous systems to develop an effective process. Batch processing demonstrated the advantageous properties of NcCO 2 as an alternative reaction medium. Three immobilized lipases (Novozym 435, Lipozyme RM IM, and Lipozyme TL IM from Novozymes) were tested, with Lipozyme TL IM the most effective, showing the highest conversion. Biodiesel conversion from several edible and non-edible oil feedstocks reached >92%. Higher conversion (99.0%) was obtained in a shorter time by employing repeated batch processes with optimized conditions: 44.3 g (500 mM) canola oil, a substrate molar ratio (methanol:oil) of 3:1, an enzyme loading of 20 wt% (of the oil used), at 30 °C, 100 bar, and 300 rpm agitation. The enzyme maintained 80.2% of its initial stability after being reused eight times. These results suggest that this method produces biodiesel energy-efficiently and environment-friendly.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-011-0637-5