Periodontal regeneration following implantation of cementum and periodontal ligament-derived cells

• Published in: Journal of periodontal research 2012-02, Vol.47 (1), p.33-44
• Main Authors: Nuñez, J., Sanz-Blasco, S., Vignoletti, F., Muñoz, F., Arzate, H., Villalobos, C., Nuñez, L., Caffesse, R. G., Sanz, M.
• Format: Article
• Language: English
• Subjects: Alveolar Bone Loss - pathology; Alveolar Bone Loss - surgery; Alveolar Process - pathology; Animals; Bicuspid - pathology; Biological and medical sciences; Cell Culture Techniques; Cell Separation; Cell Survival; Cell Transplantation; cementoblasts; Cementogenesis - physiology; Collagen; Connective Tissue - pathology; Debridement; dental cementum; Dental Cementum - cytology; Dentistry; Dogs; Guided Tissue Regeneration, Periodontal - methods; intrabony periodontal defects; Male; Medical sciences; mesenchymal stem cells; Otorhinolaryngology. Stomatology; Periodontal Ligament - cytology; periodontal regeneration; Random Allocation; Subgingival Curettage; Tissue Engineering; Tissue Scaffolds; Treatment Outcome
• ISSN: 0022-3484; 1600-0765
• DOI: 10.1111/j.1600-0765.2011.01402.x

Nuñez J, Sanz‐Blasco S, Vignoletti F, Muñoz F, Arzate H, Villalobos C, Nuñez L, Caffesse RG, Sanz M. Periodontal regeneration following implantation of cementum and periodontal ligament‐derived cells. J Periodont Res 2012; 47: 33–44. © 2011 John Wiley & Sons A/S Background and Objective:  The periodontal regeneration of bone defects is often unsatisfactory and could be largely improved by cell therapy. Therefore, the purpose of this study was to evaluate the regenerative potential of implanting canine cementum‐derived cells (CDCs) and canine periodontal ligament‐derived cells (PDLDCs) in experimentally created periodontal intrabony defects in beagle dogs. Material and Methods:  Cells were obtained from premolars extracted from four beagle dogs. Three‐wall intrabony periodontal defects, 3 mm wide and 4 mm deep, were surgically created in their second and fourth premolars and plaque was allowed to accumulate. Once the defects were surgically debrided, periodontal regeneration was attempted by random implantation of collagen sponges embedded with 750,000 CDCs, 750,000 PDLDCs or culture medium. After 3 mo of healing, specimens were obtained and periodontal regenerative outcomes were assessed histologically and histometrically. Results:  The histological analysis showed that a minimal amount of new cementum was formed in the control group (1.56 ± 0.39 mm), whereas in both test groups, significantly higher amounts of new cementum were formed (3.98 ± 0.59 mm in the CDC group and 4.07 ± 0.97 mm in the PDLDC group). The test groups also demonstrated a larger dimension of new connective tissue, resulting in a significantly more coronal level of histological attachment. Conclusion:  This proof‐of‐principle study suggests that cellular therapy, in combination with a collagen sponge, promoted periodontal regeneration in experimental intrabony periodontal defects.