Glycerol-Induced Renal Damage Improved by 7-O-Galloyl-D-sedoheptulose Treatment through Attenuating Oxidative Stress

• Published in: Biological & pharmaceutical bulletin 2012/01/01, Vol.35(1), pp.34-41
• Main Authors: Park, Chan Hum, Tanaka, Takashi, Cho, Eun Ju, Park, Jong Cheol, Shibahara, Naotoshi, Yokozawa, Takako
• Format: Article
• Language: English
• Subjects: 7-O-galloyl-D-sedoheptulose; Acute Kidney Injury - chemically induced; Acute Kidney Injury - drug therapy; Acute Kidney Injury - metabolism; acute renal failure; Animals; Antioxidants - metabolism; Antioxidants - pharmacology; Antioxidants - therapeutic use; Blood Urea Nitrogen; Corni Fructus; Cornus - chemistry; Creatinine - blood; Down-Regulation; Drugs, Chinese Herbal - pharmacology; Drugs, Chinese Herbal - therapeutic use; Fruit; Glycerol; Heptoses - pharmacology; Heptoses - therapeutic use; Inflammation Mediators - metabolism; Kidney - drug effects; Kidney - physiopathology; Lipid Peroxidation - drug effects; Male; NADPH Oxidase 4; NADPH Oxidases - metabolism; Osmolar Concentration; oxidative stress; Oxidative Stress - drug effects; Phytotherapy; Rats; Rats, Wistar; Reactive Oxygen Species - metabolism; Sodium - metabolism; Superoxides - metabolism; Urination - drug effects
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.35.34

The protective effect of 7-O-galloyl-D-sedoheptulose (GS), isolated from Corni Fructus as an active component, against acute renal failure (ARF) induced by glycerol was investigated. The administration of GS led to a decline in the levels of blood urea nitrogen and creatinine; on the other hand, it did not have a significant effect on creatinine clearance. Furthermore, GS also significantly decreased the urine volume and fractional excretion of sodium, but it increased the urine osmolarity, suggesting the protective role of GS against renal dysfunction. Oxidative stress under ARF was attenuated by GS through the inhibition of lipid peroxidation, scavenging of reactive oxygen species (ROS), and elevation of the antioxidative status. Renal oxidative stress is related to the overproduction of ROS by nicotinamide adenine dinucleotide phosphate (NAD(P)H) oxidase; therefore, in the present study, the protein expression of p22phox and NAD(P)H oxidase-4 (Nox-4) was investigated. GS down-regulated the protein expression of p22phox; on the other hand, it did not significantly affect the expression of Nox-4. This indicates that GS inhibits the production of superoxide by regulating a component of NAD(P)H oxidase, p22phox. Furthermore, GS down-regulated the expressions of nuclear factor-κB (NF-κΒ) and inducible nitric oxide (NO) synthase (iNOS), suggesting that GS protects against NO-induced inflammatory pathological conditions under ARF through the regulation of NF-κB and iNOS expressions. The present study indicates that GS exerts a protective effect against ARF through the recovery of renal dysfunction and attenuation of renal oxidative stress by regulating related protein expression.