miR-21 functionally interacts with the 3'UTR of chemokine CCL20 and down-regulates CCL20 expression in miR-21 transfected colorectal cancer cells

As deregulation of miRNAs and chemokine CCL20 was shown to play a role in colorectal cancer (CRC) pathogenesis, we analyzed the functional interactions of candidate miRNAs with CCL20 mRNA. After target prediction software programs indicated a role for miR-21 in CCL20 regulation, we applied the lucif...

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Bibliographic Details
Published in:Cancer letters 2012-03, Vol.316 (1), p.105-112
Main Authors: Vicinus, Benjamin, Rubie, Claudia, Faust, Sabrina K, Frick, Vilma Oliveira, Ghadjar, Pirus, Wagner, Mathias, Graeber, Stefan, Schilling, Martin K
Format: Article
Language:English
Subjects:
3' Untranslated Regions
Caco-2 Cells
Cell cycle
Cell Line, Tumor
Chemokine CCL20 - biosynthesis
Chemokine CCL20 - genetics
Chemokine CCL20 - metabolism
Chemokines
Colorectal Neoplasms - genetics
Colorectal Neoplasms - metabolism
Down-Regulation
Experiments
Gene expression
Humans
Ligands
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
Mutagenesis, Site-Directed - methods
RNA, Messenger - genetics
Transfection - methods
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Summary:As deregulation of miRNAs and chemokine CCL20 was shown to play a role in colorectal cancer (CRC) pathogenesis, we analyzed the functional interactions of candidate miRNAs with CCL20 mRNA. After target prediction software programs indicated a role for miR-21 in CCL20 regulation, we applied the luciferase reporter assay system to demonstrate that miR-21 functionally interacts with the 3'UTR of CCL20 mRNA and down-regulates CCL20 in miR-21 mimic transfected CRC cell lines (Caco-2, SW480 and SW620). Thus, regulation of CCL20 expression by miR-21 might be a regulatory mechanism involved in progression of CRC.
ISSN:0304-3835
1872-7980
DOI:10.1016/j.canlet.2011.10.031