Targeting Glut1-overexpressing MDA-MB-231 cells with 2-deoxy- d -g1ucose modified SPIOs

• Published in: European journal of radiology 2012-01, Vol.81 (1), p.95-99
• Main Authors: Shan, Xiu Hong, Hu, Hui, Xiong, Fei, Gu, Ning, Geng, Xin Dong, Zhu, Wei, Lin, Jiang, Wang, Ya Fei
• Format: Article
• Language: English
• Subjects: 2-Deoxy- d-glucose; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cell Line, Tumor; Contrast Media - pharmacokinetics; Deoxyglucose - pharmacokinetics; Dextrans - pharmacokinetics; Glucose transporter; Glucose Transporter Type 1 - metabolism; Humans; Magnetic resonance imaging; Magnetic Resonance Imaging - methods; Magnetite Nanoparticles; Radiology; Superparamagnetic iron oxide; Tumor; Up-Regulation
• ISSN: 0720-048X; 1872-7727
• DOI: 10.1016/j.ejrad.2011.03.013

Abstract Glucose transporter (Glut), a cellular transmembrane receptor, plays a key role in cell glucose metabolism and is linked to a poor prognosis in various human cancers. In this study, we prepared γ-Fe2 O3 NPs coated with DMSA, in which modified with 2-DG, then γ-Fe2 O3 @DMSA-DG NPs was constructed. The specific interactions between Glut1-overexpressing tumor cells (MDA-MB-231) and γ-Fe2 O3 @DMSA-DG NPs were observed using Prussian blue staining and transmission electron microscope (TEM), and found that γ-Fe2 O3 @DMSA-DG NPs were absorbed targetedly by the cells. Furthermore, the capacity of transporting SPIOs into tumor cells using these γ-Fe2 O3 @DMSA-DG NPs was evaluated with a 1.5 T clinical magnetic resonance imaging (MRI) scanner. It was found that the acquired MRI T2 signal intensity of MDA-MB-231cells that were treated with the γ-Fe2 O3 @DMSA-DG NPs decreased significantly, and it was inhibited by competition with antibody of Glut1. Our results suggest that γ-Fe2 O3 @DMSA-DG NPs are a useful targeting to Glut1-overexpressing tumor cells in vitro and that γ-Fe2 O3 @DMSA-DG NPs may serve as a MRI-targeted tumor agent for better tumor imaging.