Effect of Chinese Herbal Drug-Containing Serum for Activating- Blood and Dispelling-Toxin on ox-LDL-Induced Inflammatory Factors＇ Expression in Endothelial Cells

• Published in: Chinese journal of integrative medicine 2012, Vol.18 (1), p.30-33
• Main Authors: Jiang, Yue-rong, Miao, Yu, Yang, Lin, Xue, Mei, Guo, Chun-yu, Ma, Xiao-juan, Yin, Hui-jun, Shi, Da-zhuo, Chen, Ke-ji
• Format: Article
• Language: English
• Subjects: Animals; Capsules; Cell Membrane - drug effects; Cell Membrane - metabolism; Drugs, Chinese Herbal - pharmacology; E-Selectin - metabolism; Human Umbilical Vein Endothelial Cells - drug effects; Human Umbilical Vein Endothelial Cells - metabolism; Humans; Inflammation Mediators - metabolism; Intercellular Adhesion Molecule-1 - metabolism; Interleukin-6 - metabolism; Lipoproteins, LDL - metabolism; Medicine; Medicine & Public Health; Original Article; Rats; Rats, Wistar; Solubility - drug effects; Subcellular Fractions - drug effects; Subcellular Fractions - metabolism; Toxins, Biological - metabolism; Tumor Necrosis Factor-alpha - metabolism; 中国; 中药复方; 人脐静脉内皮细胞; 炎症因子; 细胞表达; 血清; 解毒素; 诱导
• ISSN: 1672-0415; 1993-0402
• DOI: 10.1007/s11655-011-0849-1

Objective： To investigate the effects of drug-containing serum of Chinese herbal compound, Xiongshao Capsule （芎芍胶囊, XS, for activating-blood） and Huanglian Capsule （黄连胶囊, HL, for dispellingtoxin） on the oxidized low-density lipoprotein （ox-LDL）-induced inflammatory factors in human umbilical vein endothelial cells （HUVECs）. Methods： Thirty-two rats were randomly divided into four groups： the blank control group treated with distilled water, the positive control group treated with simvastatin （1.8 mg/kg）, the test group I treated with Chinese herbal compound of XS （0.135 g/kg）, and the test group 1T treated with Chinese herbal compound of XS （0.135 g/kg） and HL （0.135 g/kg）. All the treatments were administered for 7 successive days by gastrogavage. Rats＇ blood serum was harvested 1 h after the last administration to prepare respective drug- containing serum. HUVECs were exposed to ox-LDL （100 μg/mL） to induce cell injury model and incubated with corresponding drug-containing serum for 24 h. Untreated HUVECs were set for blank control. Levels of interleukin-6 （IL-6）, tumor necrosis factor-α （TNF-α）, and soluble intercellular adhesion molecule-1 （slCAM-1） in supematant of cultured HUVECs were determined by enzyme-linked immunosorbent assay （ELISA）. HUVEC surface expressions of ICAM-1 and E-selectin were determined by flow cytometry. Results： Levels of IL-6, TNF-α, and sICAM-1 in the supernatant of HUVECs as well as the cell surface expressions of ICAM-1 and E-selectin significantly increased after 24-h ox-LDL stimulation （P〈0.01）, while the abnormal elevations, except slCAM-1 in the test group Ⅰ, were all reduced in the treated groups （the positive control and the two test groups） significantly （P〈0.01 or P〈0.05）. Besides, the effect in the test group Ⅱ seemed somewhat higher than that in the test group Ⅰ but with no statistical significance （P〉0.05）. Conclusion： Drug-containing serum of XS plus HL has a certain inhibitory effect on the vascular endothelial inflammation response induced by ox-LDL.