Vitamin D sensitive EBNA-1 specific T cells in the cerebrospinal fluid of patients with multiple sclerosis

• Published in: Journal of neuroimmunology 2011-12, Vol.240, p.87-96
• Main Authors: Lossius, Andreas, Vartdal, Frode, Holmøy, Trygve
• Format: Article
• Language: English
• Subjects: Adult; Allergy and Immunology; Blood; Cell Line; Cerebrospinal fluid; Clone Cells; EBNA-1; Epitopes, T-Lymphocyte - immunology; Epstein-Barr virus; Epstein-Barr Virus Nuclear Antigens - cerebrospinal fluid; Epstein-Barr Virus Nuclear Antigens - immunology; Female; Humans; IL-17; Lymphocyte Activation - immunology; Male; Multiple sclerosis; Multiple Sclerosis - cerebrospinal fluid; Multiple Sclerosis - immunology; Multiple Sclerosis - virology; Neurology; T-Lymphocyte Subsets - immunology; T-Lymphocyte Subsets - pathology; T-Lymphocyte Subsets - virology; Vitamin D; Vitamin D - analogs & derivatives; Vitamin D - pharmacology; Young Adult
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/j.jneuroim.2011.09.011

Abstract The pathogenesis of multiple sclerosis (MS) may involve intrathecal Epstein–Barr virus nuclear antigen-1 (EBNA-1) specific T cells susceptible to modulation by vitamin D. We established EBNA-1 reactive T cell lines from the cerebrospinal fluid (CSF) and blood of three MS patients and cloned EBNA-1 specific CD4+ T cells from two of these. T cell clones from CSF and blood displayed Th1 or Th17 phenotypes and were restricted by HLA-DR molecules, in one patient encoded by the DRB1*0403 or DRB1*1501 haplotypes. 1,25-dihydroxyvitamin D inhibited proliferation and suppressed secretion of IFN-γ and IL-17, irrespective of T cell origin and HLA restriction.