Phosphorus magnetic resonance spectroscopy in malformations of cortical development

• Published in: Epilepsia (Copenhagen) 2011-12, Vol.52 (12), p.2276-2284
• Main Authors: Andrade, Celi S., Otaduy, Maria C. G., Valente, Kette D. R., Maia, Danilo F., Park, Eun J., Valério, Rosa M. F., Tsunemi, Miriam H., Leite, Claudia C.
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphate; Adolescent; Adult; ATP; Bioenergetics; Biological and medical sciences; Brain; Brain - metabolism; Brain - pathology; Child; Cortex; Data processing; Energetics; Epilepsy; Female; Follow-Up Studies; Glycerylphosphorylcholine; Headache. Facial pains. Syncopes. Epilepsia. Intracranial hypertension. Brain oedema. Cerebral palsy; Humans; Image Processing, Computer-Assisted - methods; Lipid metabolism; Magnesium; Magnesium - metabolism; Magnetic resonance imaging; Magnetic Resonance Spectroscopy; Male; Malformations of cortical development; Malformations of Cortical Development - diagnosis; Malformations of Cortical Development - metabolism; Malformations of the nervous system; Medical research; Medical sciences; Membrane turnover; Middle Aged; Nervous system (semeiology, syndromes); Neurology; Neurometabolism; NMR; Nuclear magnetic resonance; pH effects; Phosphate; phosphocholine; Phosphocreatine; phosphodiesters; Phospholipids; phosphomonoesters; Phosphoric Diester Hydrolases; Phosphoric Monoester Hydrolases; Phosphorus; Phosphorus - metabolism; Phosphorus Isotopes; Phosphorus spectroscopy; Seizures; Spectrum analysis; Statistics; Young Adult
• ISSN: 0013-9580; 1528-1167
• DOI: 10.1111/j.1528-1167.2011.03281.x

Summary Purpose:  The aim of this study was to evaluate phospholipid metabolism in patients with malformations of cortical development (MCDs). Methods:  Thirty‐seven patients with MCDs and 31 control subjects were studied using three‐dimensional phosphorus magnetic resonance spectroscopy (31P‐MRS) at 3.0 T. The voxels in the lesions and in the frontoparietal cortex of the control subjects were compared (the effective volumes were 12.5 cm3). Robust quantification methods were applied to fit the time‐domain data to the following resonances: phosphoethanolamine (PE); phosphocholine (PC); inorganic phosphate (Pi); glycerophosphoethanolamine (GPE); glycerophosphocholine (GPC); phosphocreatine (PCr); and α‐, β‐, and γ‐adenosine triphosphate (ATP). We also estimated the total ATP (ATPt = α‐+β‐+γ‐ATP), phosphodiesters (PDE = GPC+GPE), phosphomonoesters (PME = PE+PC), and the PME/PDE, PCr/ATPt and PCr/Pi ratios. The magnesium (Mg2+) levels and pH values were calculated based on PCr, Pi, and β‐ATP chemical shifts. Key Findings:  Compared to controls and assuming that a p‐value