Emulsive Polymerization as a Method of Enzyme Modification Preserving Biological Properties of Nanostructures

The proposed method of emulsive polymerization provides the possibility of modifying and obtaining insoluble forms of superoxide dismutase (SOD), glutathione reductase, and streptolysin-O preserving nanoobjects (conformationally active centers and antigenic determinants) in their native states. Apar...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2011-04, Vol.150 (6), p.765-769
Main Authors: Gontar, I. P., Sycheva, G. F., Alexandrov, A. V., Shilova, L. N., Simakova, E. S., Emelyanov, N. N., Matasova, N. N., Maslakova, L. A., Zborovsky, A. B.
Format: Article
Language:English
Subjects:
Antigenic determinants
Antioxidants - analysis
Bacterial Proteins - chemistry
Bacterial Proteins - metabolism
Biomedical and Life Sciences
Biomedicine
Cell Biology
Enzymes
Glutathione Reductase - chemistry
Glutathione Reductase - metabolism
Humans
Hydrogen
Internal Medicine
Laboratory Medicine
Magnetic properties
Methods
Nanostructures
Pathology
Peroxides - analysis
Polyacrylamide
Polymerization
Streptolysins - chemistry
Streptolysins - metabolism
Superoxide
Superoxide Dismutase - chemistry
Superoxide Dismutase - metabolism
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Summary:The proposed method of emulsive polymerization provides the possibility of modifying and obtaining insoluble forms of superoxide dismutase (SOD), glutathione reductase, and streptolysin-O preserving nanoobjects (conformationally active centers and antigenic determinants) in their native states. Apart from enzymatic and immunological properties, the samples acquired some new features: resistance to high temperature, resistance to 3 M КCNS solution and buffer solutions with high concentration of hydrogen ions, and resistance to preserving solutions. Magnetic properties provide the possibility of simplifying enzyme-linked immunosorbent and immunofluorescence assays. In addition, sensitivity of these assays was by an order of magnitude higher and the specificity fully preserved. Taking all the facts into account, we prepared agents for long-term and repeated use. Due to preserved enzymatic properties, insoluble forms of SOD and glutathione reductase can be considered as a tool for correction of peroxide-antioxidant balance and associated immunological abnormalities.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-011-1244-9