Interaction of 5-Fluorouracil Loaded Nanoparticles with 1,2-Dimyristoyl-sn-glycero-3-phosphocholine Liposomes Used as a Cellular Membrane Model

Nuclear magnetic resonance (NMR) spectroscopy and steady-state fluorescence anisotropy were used to study the behavior and interaction of 5-fluorouracil, both in a free form (5FU) and included in the polymer matrix of poly(butylcyanoacrylate) nanoparticles (5FUPBCN) with a phospholipid bilayer of la...

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Bibliographic Details
Published in:The journal of physical chemistry. B 2012-01, Vol.116 (1), p.667-675
Main Authors: Lopes, Silvia, Simeonova, Margarita, Gameiro, Paula, Rangel, Maria, Ivanova, Galya
Format: Article
Language:English
Subjects:
Aqueous environments
B: Biophysical Chemistry
Dimyristoylphosphatidylcholine - chemistry
Drugs
Fluorescence Polarization
Fluorouracil - chemistry
Lipid Bilayers - chemistry
Liposomes
Liposomes - chemistry
Magnetic Resonance Spectroscopy
Membranes
Models, Molecular
Nanoparticles
Nanoparticles - chemistry
Nuclear magnetic resonance
Phospholipids
Vesicles
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Summary:Nuclear magnetic resonance (NMR) spectroscopy and steady-state fluorescence anisotropy were used to study the behavior and interaction of 5-fluorouracil, both in a free form (5FU) and included in the polymer matrix of poly(butylcyanoacrylate) nanoparticles (5FUPBCN) with a phospholipid bilayer of large unilammellar vesicles composed of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), as a model system of biomembranes. The results confirm an interaction and penetration of 5FU into the phospholipid bilayer of DMPC liposomes. Different mechanisms of drug transfer from the aqueous environment into the model membrane environment, for the free drug and that incorporated into polymer nanoparticles, are suggested: (i) concentration-dependent reversible diffusion of the free 5FU and (ii) sustained 5FU release from nanoparticles adsorbed on the liposome surface resulting in continuous delivery of the drug into the phospholipid bilayers of the DMPC liposomes.
ISSN:1520-6106
1520-5207
DOI:10.1021/jp210088n