Immobilization of fluorous oligosaccharide recognized by influenza virus on polytetrafluoroethylene filter

The lactoside with PEG-fluorous tag was introduced to BHK-21(C-13) cells to generate a GM3-type oligosaccharide (Siaα2-3Galβ1-4Glc). The GM3-type oligosaccharide obtained was easily immobilized by spotting onto commercially available polytetrafluoroethylene (PTFE) filter through non-covalent fluorou...

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Bibliographic Details
Published in:Bioorganic & medicinal chemistry letters 2012-01, Vol.22 (2), p.1251-1254
Main Authors: Tojino, Mami, Mori, Masako, Kasuya, Maria Carmelita Z., Hatanaka, Kenichi, Kawaguchi, Atsushi, Nagata, Kyosuke, Shirai, Takashi, Mizuno, Mamoru
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Line
Cricetinae
Fluorous
Influenza A virus - chemistry
Influenza virus
Medical sciences
membrane proteins
Membranes, Artificial
Oligosaccharide
oligosaccharides
Oligosaccharides - chemistry
Orthomyxoviridae
Pharmacology. Drug treatments
Polyethylene Glycols - chemistry
polytetrafluoroethylene
Polytetrafluoroethylene - chemistry
Polytetrafluoroethylene filter
Saccharide primer
sialic acids
viruses
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Summary:The lactoside with PEG-fluorous tag was introduced to BHK-21(C-13) cells to generate a GM3-type oligosaccharide (Siaα2-3Galβ1-4Glc). The GM3-type oligosaccharide obtained was easily immobilized by spotting onto commercially available polytetrafluoroethylene (PTFE) filter through non-covalent fluorous affinity and simply assessed by dot blot method using the interaction of carbohydrate- with proteins which recognize sialic acid such as virus membrane proteins.
ISSN:0960-894X
1464-3405
DOI:10.1016/j.bmcl.2011.11.057