Action of ANP on the nongenomic dose-dependent biphasic effect of aldosterone on NHE1 in proximal S3 segment

• Published in: The Journal of steroid biochemistry and molecular biology 2012-02, Vol.128 (3-5), p.89-97
• Main Authors: Braga-Sobrinho, C., Leite-Dellova, D.C.A., Mello-Aires, M.
• Format: Article
• Language: English
• Subjects: [Ca2+]i; Acid-Base Equilibrium - drug effects; Aldosterone; Aldosterone - metabolism; Ammonium Chloride - toxicity; Animals; ANP; Atrial Natriuretic Factor - metabolism; Calcium Signaling - drug effects; Chelating Agents - pharmacology; Hydrogen-Ion Concentration; In Vitro Techniques; Kidney Tubules, Proximal - drug effects; Kidney Tubules, Proximal - metabolism; Kinetics; Male; Microdissection; Mifepristone - pharmacology; Mineralocorticoid Receptor Antagonists - pharmacology; NHE1; Osmolar Concentration; pHi; Proximal tubule; Rats; Rats, Wistar; Receptors, Glucocorticoid - antagonists & inhibitors; Sodium-Hydrogen Exchanger 1; Sodium-Hydrogen Exchangers - metabolism; Spironolactone - pharmacology
• ISSN: 0960-0760; 1879-1220
• DOI: 10.1016/j.jsbmb.2011.11.011

► Aldosterone stimulated/impaired the Na+/H+ exchanger in S3 segments. ► The intracellular pH recovery rate and cytosolic free calcium concentration were investigated by using the fluorescent probes BCECF-AM and FLUO-4-AM. ► The nongenomicaldosterone effects on NHE1 are affected by ANP, RU 486 and BAPTA. ► The intracellular Ca2+ regulates the NHE1 activity. The rapid (2min) nongenomic effects of aldosterone (ALDO) and/or spironolactone (MR antagonist), RU 486 (GR antagonist), atrial natriuretic peptide (ANP) and dimethyl-BAPTA (BAPTA) on the intracellular pH recovery rate (pHirr) via NHE1 (basolateral Na+/H+ exchanger isoform), after the acid load induced by NH4Cl, and on the cytosolic free calcium concentration ([Ca2+]i) were investigated in the proximal S3 segment isolated from rats, by the probes BCECF-AM and FLUO-4-AM, respectively. The basal pHi was 7.15±0.008 and the basal pHirr was 0.195±0.012pHunits/min (number of tubules/number of tubular areas=16/96). Our results confirmed the rapid biphasic effect of ALDO on NHE1: ALDO (10−12M) increases the pHirr to approximately 59% of control value, and ALDO (10−6M) decreases it to approximately 49%. Spironolactone did not change these effects, but RU 486 inhibited the stimulatory effect and maintained the inhibitory effect. ANP (10−6M) or BAPTA (5×10−5M) alone had no significant effect on NHE1 but prevented both effects of ALDO on this exchanger. The basal [Ca2+]i was 104±3nM (15), and ALDO (10−12 or 10−6M) increased the basal [Ca2+]i to approximately 50% or 124%, respectively. RU 486, ANP and BAPTA decreased the [Ca2+]i and inhibited the stimulatory effect of both doses of ALDO. The results suggest the involvement of GR on the nongenomic effects of ALDO and indicate a pHirr-regulating role for [Ca2+]i that is mediated by NHE1, stimulated/impaired by ALDO, and affected by ANP or BAPTA with ALDO. The observed nongenomic hormonal interaction in the S3 segment may represent a rapid and physiologically relevant regulatory mechanism in the intact animal under conditions of volume alterations.