Adoptive Transfer of Dendritic Cells Pulsed With Fasciola hepatica Antigens and Lipopolysaccharides Confers Protection Against Fasciolosis in Mice

Dendritic cells (DCs) can function as adjuvants able to mediate protection against different pathogens. Given that successful vaccination against Fasciola hepatica is mostly related to the induction of Th1 responses, we studied the potential of DCs loaded with F. hepatica antigens and lipopolysaccha...

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Bibliographic Details
Published in:The Journal of infectious diseases 2012-02, Vol.205 (3), p.506-514
Main Authors: Falcón, Cristian R., Carranza, Franco A., Aoki, Pilar, Motrán, Claudia C., Cervi, Laura
Format: Article
Language:English
Subjects:
Adoptive Transfer - methods
Animals
Antigens
Antigens, Helminth - immunology
Biological and medical sciences
Cytokines
Cytokines - secretion
Dendritic cells
Dendritic Cells - immunology
Disease Models, Animal
Diseases caused by trematodes
Distomatoses
Fasciola hepatica - immunology
Fascioliasis - immunology
Fascioliasis - pathology
Fascioliasis - prevention & control
Female
Fundamental and applied biological sciences. Psychology
Helminthic diseases
Histocytochemistry
Infections
Infectious diseases
Lipopolysaccharides
Lipopolysaccharides - immunology
Liver
Liver - immunology
Liver - pathology
Medical sciences
Mice
Mice, Inbred BALB C
Microbiology
Microscopy
Parasites
Parasitic diseases
Splenocytes
Th1 Cells - immunology
Vaccination
Vaccination - methods
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Summary:Dendritic cells (DCs) can function as adjuvants able to mediate protection against different pathogens. Given that successful vaccination against Fasciola hepatica is mostly related to the induction of Th1 responses, we studied the potential of DCs loaded with F. hepatica antigens and lipopolysaccharide (LPS) (which promote DCs maturation) as a vaccine against fasciolosis in BALB/c mice. However, only a semimature phenotype was achieved when DCs were simultaneously cultured with an F. hepatica total extract (TE) and LPS. The activation status of TE-loaded DCs was enhanced when these cells were treated with TE 90 minutes before being stimulated with LPS (TE90 DCs). More importantly, a single vaccination of mice with TE90 DCs stimulated a systemic Th1 response and conferred protection against hepatic damage induced by F. hepatica infection.Thus, TE90 DCs may prove to be a useful new tool for vaccination against F. hepatica.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/jir606