Evaluation of conventional molecular diagnosis of Mycobacterium tuberculosis in clinical specimens from Morocco

Tuberculosis is a major public health threat, annually affecting new individuals worldwide, especially those in developing countries. Rapid detection of the agent and effective treatment are two important factors in controlling this disease. The present study aimed to evaluate polymerase chain react...

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Bibliographic Details
Published in:Journal of infection in developing countries 2012-01, Vol.6 (1), p.40-45
Main Authors: Zakham, Fathiah, Bazoui, Halima, Akrim, Mohammed, Lemrabet, Sanae, Lahlou, Ouafae, Elmzibri, Mohamed, Benjouad, Abdelaziz, Ennaji, My Mustapha, Elaouad, Rajae
Format: Article
Language:English
Subjects:
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Base Sequence
Cerebrospinal Fluid - microbiology
Chaperonin 60 - chemistry
Chaperonin 60 - genetics
Humans
Molecular Sequence Data
Morocco
Mycobacterium tuberculosis - classification
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - isolation & purification
Polymerase Chain Reaction - methods
Predictive Value of Tests
Sensitivity and Specificity
Sequence Analysis, DNA
Sputum - microbiology
Tuberculosis
Tuberculosis, Pulmonary - diagnosis
Tuberculosis, Pulmonary - microbiology
Tuberculosis, Spinal - diagnosis
Tuberculosis, Spinal - microbiology
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Summary:Tuberculosis is a major public health threat, annually affecting new individuals worldwide, especially those in developing countries. Rapid detection of the agent and effective treatment are two important factors in controlling this disease. The present study aimed to evaluate polymerase chain reaction (PCR) as a rapid and direct molecular method for the diagnosis of Mycobacterium tuberculosis (MTB) in 70 clinical specimens (62 sputum samples, six cerebrospinal fluids, and two biopsies) using heat shock protein (hsp65) as the gene target. Automated sequencing of the same gene was used for the identification of MTB to the species level. The sensitivity of PCR was 81.13%, with specificity of 88.24%; the positive and negative predictive values were 95.56% and 60%, respectively. Based on these results, the hsp65 gene sequence can be used to differentiate the members of MTB complex from non-tuberculosis mycobacteria (NTM).
ISSN:1972-2680
2036-6590
1972-2680
DOI:10.3855/jidc.1857