Native polyubiquitin promoter of rice provides increased constitutive expression in stable transgenic rice plants

The rice Ubiquitin1 (Ubi1) promoter was tested to evaluate its capacity to express the heterologous gusA gene encoding β-glucuronidase in transgenic rice tissue relative to the commonly used Ubi1 corn promoter and the rice gibberellic acid insensitive (GAI) gene promoter element. Experimental result...

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Bibliographic Details
Published in:Plant cell reports 2012-02, Vol.31 (2), p.271-279
Main Authors: Bhattacharyya, Jagannath, Chowdhury, Asif Hasan, Ray, Samarjit, Jha, Jyoti Krishna, Das, Srirupa, Gayen, Srimonta, Chakraborty, Anirban, Mitra, Joy, Maiti, Mrinal K, Basu, Asitava, Sen, Soumitra K
Format: Article
Language:English
Subjects:
Analysis of Variance
beta -Glucuronidase
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Blotting, Southern
Blotting, Western
Cell Biology
Cloning, Molecular
Corn
Crosses, Genetic
DNA, Plant - genetics
Fluorometry
Fundamental and applied biological sciences. Psychology
gene expression
Gene Expression Regulation, Plant
Gibberellic acid
Glucuronidase - genetics
Glucuronidase - metabolism
GusA gene
Life Sciences
Original Paper
Oryza - genetics
Oryza sativa
Plant Biochemistry
Plant Sciences
Plants, Genetically Modified
Polyubiquitin - genetics
promoter regions
Promoter Regions, Genetic - genetics
Promoters
rice
Transgenic plants
Zea mays - metabolism
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Summary:The rice Ubiquitin1 (Ubi1) promoter was tested to evaluate its capacity to express the heterologous gusA gene encoding β-glucuronidase in transgenic rice tissue relative to the commonly used Ubi1 corn promoter and the rice gibberellic acid insensitive (GAI) gene promoter element. Experimental results showed increased expression of gusA gene in rice tissue when driven by the native Ubi1 promoter when compared to the use of corn Ubi1 promoter. Results further indicated that the cis-regulatory elements present in the native promoter element might have been responsible for high expression. However, the gusA gene expression level when driven by the rice GAI promoter was notably lower than both Ubi1 promoters. The present study, thus, for the first time helped to demonstrate that the native Ubi1 promoter is a promising genetic element in transgenic approaches for constitutive expression of any gene in rice tissue.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-011-1161-4