Plasma insulin-like peptide 3 and testosterone concentrations in male dogs: Changes with age and effects of cryptorchidism

• Published in: Theriogenology 2012-02, Vol.77 (3), p.550-557
• Main Authors: Pathirana, I.N, Yamasaki, H, Kawate, N, Tsuji, M, Büllesbach, E.E, Takahashi, M, Hatoya, S, Inaba, T, Tamada, H
• Format: Article
• Language: English
• Subjects: Aging - physiology; Animals; blood; Cryptorchidism; Cryptorchidism - blood; Cryptorchidism - veterinary; detection limit; disease diagnosis; Dog; Dog Diseases - blood; Dog Diseases - metabolism; Dogs; elderly; fluorescence; Gene Expression Regulation - physiology; immunoassays; Insulin - blood; Insulin - metabolism; Male; Orchiectomy - veterinary; Plasma INSL3 concentration; Proteins - metabolism; testes; Testis; Testosterone; Testosterone - blood; Testosterone - metabolism; Time-resolved fluorescence immunoassay
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2011.08.030

The objectives were to: (1) develop a time-resolved fluorescence immunoassay (TRFIA) to measure insulin-like peptide 3 (INSL3) in canine plasma; (2) investigate changes of plasma concentrations of INSL3 and testosterone with age in normal male dogs; and (3) compare hormonal concentrations among cryptorchid, normal, and castrated dogs to evaluate endocrine function of the Leydig cell component in retained testes. Blood samples were taken from normal male dogs from prepubertal age to advanced age (4 mo to 14 y, n = 89), and from unilateral cryptorchid (n = 31), bilateral cryptorchid (n = 7), and castrated dogs (n = 3). Canine plasma INSL3 was measured with a newly developed TRFIA. The minimum detection limit of the INSL3 assay was 0.02 ng/ml and the detection range was 0.02 to 20 ng/ml. Plasma INSL3 concentrations increased (P < 0.05) from prepubertal age (4–6 mo) to pubertal age (6–12 mo), and then declined (P < 0.05) from pubertal age to post-pubertal age (1–5 y), reaching a plateau. Plasma testosterone concentrations increased (P < 0.0001) dramatically from prepubertal to pubertal ages, and then seemed to plateau. Concentrations of both INSL3 and testosterone were lower (P < 0.0001 for each) in bilateral cryptorchid dogs than in normal and unilateral cryptorchid dogs. The INSL3 (range: 0.05–0.43 ng/ml) and testosterone (range: 0.10–0.94 ng/ml) concentrations were readily detected in bilateral cryptorchids, but not in castrated dogs (INSL3 < 0.02 ng/ml; testosterone < 0.04 ng/ml). In conclusion, plasma INSL3 concentrations in male dogs measured by a newly developed TRFIA had a transient surge at a pubertal age, whereas testosterone did not. Lower plasma concentrations of INSL3 and testosterone in bilateral cryptorchid dogs suggest impaired endocrine functions of Leydig cell component in paired retained testes. Therefore, peripheral plasma INSL3 and testosterone concentrations have potential diagnostic value in predicting the presence of bilaterally retained testes in male dogs.