An experimental approach towards the development of an in vitro cortical-thalamic co-culture model

In this paper, we propose an experimental approach to develop an in vitro dissociated cortical-thalamic co-culture model using a dual compartment neurofluidic device. The device has two compartments separated by 10 μm wide and 3 μm high microchannels. The microchannels provide a physical isolation o...

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Bibliographic Details
Main Authors: Kanagasabapathi, Thirukumaran T, Massobrio, Paolo, Tedesco, Mariateresa, Martinoia, Sergio, Wadman, Wytse J, Decre, Michel M J
Format: Conference Proceeding
Language:English
Subjects:
Electrodes
Electrophysiology
Firing
In vitro
Microchannel
Neurons
Neuroscience
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Summary:In this paper, we propose an experimental approach to develop an in vitro dissociated cortical-thalamic co-culture model using a dual compartment neurofluidic device. The device has two compartments separated by 10 μm wide and 3 μm high microchannels. The microchannels provide a physical isolation of neurons allowing only neurites to grow between the compartments. Long-term viable co-culture was maintained in the compartmented device, neurite growth through the microchannels was verified using immunofluorescence staining, and electrophysiological recordings from the co-culture system was investigated. Preliminary analysis of spontaneous activities from the co-culture shows a distinctively different firing pattern associated with cultures of individual cell types and further analysis is proposed for a deeper understanding of the dynamics involved in the network connectivity in such a co-culture system.
ISSN:1094-687X
1558-4615
2694-0604
DOI:10.1109/IEMBS.2011.6090144