Connecting μ-fluidics to electron microscopy

A versatile methodology for electron microscopy (EM) grid preparation enabling total content sample analysis is presented. A microfluidic-dialysis conditioning module to desalt or mix samples with negative stain solution is used, combined with a robotic writing table to micro-pattern the EM grids. T...

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Bibliographic Details
Published in:Journal of structural biology 2012, Vol.177 (1), p.128-134
Main Authors: Kemmerling, Simon, Ziegler, Jörg, Schweighauser, Gabriel, Arnold, Stefan A., Giss, Dominic, Müller, Shirley A., Ringler, Philippe, Goldie, Kenneth N., Goedecke, Nils, Hierlemann, Andreas, Stahlberg, Henning, Engel, Andreas, Braun, Thomas
Format: Article
Language:English
Subjects:
AM 6.5
ammonium molybdate at pH 6.5
Animals
apoferritin
Baby Hamster Kidney
BHK
Cells, Cultured
Cricetinae
ddH 2O
double-distilled water
electron microscopy
electron tomography
Mass and shape
mass spectrometry
mass-per-length
Microfluidics
Microfluidics - instrumentation
Microscopy, Electron, Scanning Transmission - methods
MPL
NanoV 8.0
NanoVan® at pH 8.0
NanoW 6.8
NanoW® at pH 6.8
phosphotungstic acid at pH 7.0
PTA 7.0
scanning transmission electron microscopy
Single-molecule analysis
Staining and Labeling
standard deviation
STEM
Systems biology
Systems Biology - methods
TEM
TMV
tobacco mosaic virus
transmission electron microscopy
UA 4.5
UA 7.0
uranyl acetate at pH 4.5
uranyl acetate at pH 7.0
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Description
Summary:A versatile methodology for electron microscopy (EM) grid preparation enabling total content sample analysis is presented. A microfluidic-dialysis conditioning module to desalt or mix samples with negative stain solution is used, combined with a robotic writing table to micro-pattern the EM grids. The method allows heterogeneous samples of minute volumes to be processed at physiological pH for structure and mass analysis, and allows the preparation characteristics to be finely tuned.
ISSN:1047-8477
1095-8657
DOI:10.1016/j.jsb.2011.11.001