Immunogenic compositions assembled from tobacco mosaic virus-generated spherical particle platforms and foreign antigens

We reported recently that RNA-free spherical particles (SPs) generated by thermal remodelling of tobacco mosaic virus (TMV) are capable of binding GFP to their surface. Here, we show that SPs represent a universal particle platform that can form compositions by binding a diversity of various foreign...

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Bibliographic Details
Published in:Journal of general virology 2012-02, Vol.93 (Pt 2), p.400-407
Main Authors: Karpova, Olga, Nikitin, Nikolai, Chirkov, Sergey, Trifonova, Ekaterina, Sheveleva, Anna, Lazareva, Ekaterina, Atabekov, Joseph
Format: Article
Language:English
Subjects:
Antigens, Viral - immunology
Antigens, Viral - metabolism
coat proteins
Drug Carriers - chemistry
electron microscopy
epitopes
formaldehyde
glycoproteins
hemagglutinins
immune response
Immunohistochemistry
Microscopy, Immunoelectron
Orthomyxoviridae
Plum pox virus
Potato virus X
Protein Binding
recombinant proteins
Rubella virus
tobacco
Tobacco mosaic virus
Tobacco Mosaic Virus - chemistry
Virion - chemistry
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Summary:We reported recently that RNA-free spherical particles (SPs) generated by thermal remodelling of tobacco mosaic virus (TMV) are capable of binding GFP to their surface. Here, we show that SPs represent a universal particle platform that can form compositions by binding a diversity of various foreign proteins/epitopes of viral and non-viral origin to their surface. Numerous molecules of a foreign protein linked to the SP surface were revealed by immunogold electron microscopy. Several SP-based compositions were obtained containing one of the following foreign antigens: antigenic determinant A of rubella virus E1 glycoprotein; a recombinant protein containing the M2e epitope of influenza virus A protein M2; a recombinant antigen consisting of three epitopes of influenza virus A haemagglutinin; potato virus X (PVX) coat protein (CP); BSA; and PVX CP fused with the epitope of plum pox virus CP. The ‘mixed’ compositions could be also assembled by binding two different foreign antigens to each of the SPs. Immunogenicity of foreign antigens adsorbed or linked covalently to SPs in the SP-based compositions was examined. The antigenic specificity of foreign antigens was retained, whereas their immunogenicity increased significantly. It was inferred that SPs exhibit immunopotentiating activity, in particular in the form of compositions comprising SP and foreign antigen linked covalently to their surface by formaldehyde.
ISSN:1465-2099
0022-1317
1465-2099
DOI:10.1099/vir.0.036293-0