Treatment with the glucocorticoid antagonist RU486 reduces cooperative cleaning visits of a common reef fish, the lined bristletooth

• Published in: Hormones and behavior 2012, Vol.61 (1), p.37-43
• Main Authors: Ros, Albert F.H., Vullioud, Philippe, Bshary, Redouan
• Format: Article
• Language: English
• Subjects: Animal behavior; Animals; Antagonist drugs; Behavior, Animal - drug effects; Behavior, Animal - physiology; Behavioral psychophysiology; Biological and medical sciences; Cleaning mutualism; Cooperative Behavior; Cortisol; Fish; Fundamental and applied biological sciences. Psychology; Hormone Antagonists - pharmacology; Hormones; Hormones and behavior; Mifepristone; Mifepristone - pharmacology; Perciformes - parasitology; Perciformes - physiology; Psychology. Psychoanalysis. Psychiatry; Psychology. Psychophysiology; Receptors, Glucocorticoid - physiology; RU486; Stress; Stress, Physiological - drug effects; Stress, Physiological - physiology; Teleost
• ISSN: 0018-506X; 1095-6867
• DOI: 10.1016/j.yhbeh.2011.09.013

Cooperation often involves a conflict of interest. This is particularly true in situations where one individual seeks out a service but cannot properly control the quality of the service given by the partner who would gain from defecting. An example is cleaning mutualism involving the bluestreak cleaner wrasse ( Labroides dimidiatus) and its reef-fish ‘clients’. These cleaners may reduce the stress experienced by their clients by removing parasites; however they occasionally cheat clients (i.e. defect) by eating mucus and other living tissues. Here we present experimental support for the hypothesis that stress responses increase the motivation for clients to seek out such risky asymmetric interactions. We manipulated the stress response by blocking glucocorticoid receptors with the antagonist RU486 in a species that is a regular visitor of cleaner fish, the lined bristletooth ( Ctenochaetus striatus). Field observations 1 week after treatment with RU486 showed that antagonist treatment led to a reduction in cleaning duration compared to control treatment. This was not explained by a general effect on client behavior as intraspecific social behavior appeared unaffected. We propose that antagonist treatment reduced stress responses to the presence of ectoparasites, which in turn reduced the client's perception of benefits from seeking out cleaning interactions. The results demonstrate a hitherto overlooked variable role of stress and stress responses on cooperative behavior. ► We tested the effect of a glucocorticoid antagonist RU486 on inter-specific cleaning interactions. ► Cleaning interactions in reef ecosystems may relieve stress of clients because cleaners remove parasites. ► Treatment with the antagonist reduced the number of cleaning interactions in a common reef fish. ► Reducing stress responses may result in clients to perceive benefits from seeking cleaning interactions low.